Enabling biocatalysis in high‐concentration organic cosolvent by enzyme gate engineering
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DOI: 10.1002/bit.28014
Abstract: Biocatalysis in high‐concentration organic solvents (OSs) offers many advantages, but realizing this process remains a huge challenge. An R‐selective ω‐amine transaminase variant (AcATAM2) exhibited high activity toward 50 g/L pro‐sitagliptin ketone 1‐[1‐piperidinyl]‐4‐[2,4,5‐trifluorophenyl]‐1,3‐butanedione (PTfpB). However, AcATAM2 displayed… read more here.
Keywords: enzyme; concentration organic; biocatalysis high; dmso ... See more keywords