Articles with "biochemical assays" as a keyword



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In Vitro Biochemical Assays for O‐GlcNAc‐Processing Enzymes

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Published in 2017 at "ChemBioChem"

DOI: 10.1002/cbic.201700138

Abstract: O‐GlcNAc transferase (OGT) and O‐GlcNAcase (OGA) are the only enzymes that regulate the dynamics of protein O‐GlcNAcylation. Protein O‐GlcNAcylation is an important post‐translational modification (PTM) of nuclear and cytoplasmic proteins with O‐linked β‐N‐acetyl‐glucosamine (O‐GlcNAc). O‐GlcNAc… read more here.

Keywords: assays glcnac; processing enzymes; biochemical assays; vitro biochemical ... See more keywords
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Visualizing molecules of functional human profilin

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Published in 2022 at "eLife"

DOI: 10.1101/2021.09.01.458557

Abstract: Profilin-1 (PFN1) is a cytoskeletal protein that regulates the dynamics of actin and microtubule assembly. Thus, PFN1 is essential for the normal division, motility, and morphology of cells. Unfortunately, conventional fusion and direct labeling strategies… read more here.

Keywords: molecules functional; biochemical assays; pfn1; visualizing molecules ... See more keywords