Purification, biochemical and biophysical characterization of an acidic α-galactosidase from the seeds of Annona squamosa (custard apple).
Sign Up to like & getrecommendations! Published in 2021 at "International journal of biological macromolecules"
DOI: 10.1016/j.ijbiomac.2021.01.179
Abstract: Alpha galactosidase is an exoglycosidase that cleaves α-D-galactose and has numerous applications in medicine, biotechnology, food and pharma industries. In this study, a low molecular weight acidic α-galactosidase was identified from the seeds of custard… read more here.
Keywords: biochemical biophysical; acidic galactosidase; galactosidase; custard apple ... See more keywords
Development of a biochemical and biophysical suite for integral membrane protein targets: A review.
Sign Up to like & getrecommendations! Published in 2019 at "Protein expression and purification"
DOI: 10.1016/j.pep.2019.105545
Abstract: The generation of integral membrane proteins (IMPs) in heterologous systems and their characterization remains a major challenge in biomedical research. Significant efforts have been invested both in academia and in the pharmaceutical industry to establish… read more here.
Keywords: integral membrane; biochemical biophysical; biophysical suite; suite integral ... See more keywords
Comprehensive Biochemical and Biophysical Characterization of Hemoglobin-Based Oxygen Carrier Therapeutics: All HBOCs Are Not Created Equally.
Sign Up to like & getrecommendations! Published in 2018 at "Bioconjugate chemistry"
DOI: 10.1021/acs.bioconjchem.8b00093
Abstract: The development of hemoglobin (Hb)-based oxygen carriers (HBOCs) has been hampered because of safety concerns in humans. Chemical and/or genetic modifications of the Hb introduce varied structural and conformational constraint on the molecule that resulted… read more here.
Keywords: oxygen; hemoglobin based; biochemical biophysical; comprehensive biochemical ... See more keywords
Alanine Scanning Effects on the Biochemical and Biophysical Properties of Intrinsically Disordered Proteins: A Case Study of the Histidine to Alanine Mutations in Amyloid-β42
Sign Up to like & getrecommendations! Published in 2019 at "Journal of chemical information and modeling"
DOI: 10.1021/acs.jcim.8b00926
Abstract: Alanine scanning is a tool in molecular biology that is commonly used to evaluate the contribution of a specific amino acid residue to the stability and function of a protein. Additionally, this tool is also… read more here.
Keywords: alanine; histidine alanine; biochemical biophysical; biophysical properties ... See more keywords
Insights into the regulatory function of the ɛ subunit from bacterial F-type ATP synthases: a comparison of structural, biochemical and biophysical data
Sign Up to like & getrecommendations! Published in 2018 at "Open Biology"
DOI: 10.1098/rsob.170275
Abstract: ATP synthases catalyse the formation of ATP, the most common chemical energy storage unit found in living cells. These enzymes are driven by an electrochemical ion gradient, which allows the catalytic evolution of ATP by… read more here.
Keywords: atp; atp hydrolysis; biochemical biophysical; atp synthases ... See more keywords
Biochemical, biophysical, and immunological characterization of respiratory secretions in severe SARS-CoV-2 infections.
Sign Up to like & getrecommendations! Published in 2022 at "JCI insight"
DOI: 10.1172/jci.insight.152629
Abstract: Thick, viscous respiratory secretions are a major pathogenic feature of COVID-19, but the composition and physical properties of these secretions are poorly understood. We characterized the composition and rheological properties (i.e., resistance to flow) of… read more here.
Keywords: characterization respiratory; biophysical immunological; biochemical biophysical; immunological characterization ... See more keywords
Biochemical, biophysical, and functional characterisation of the E3 ubiquitin ligase APC/C regulator CDC20 from Arabidopsis thaliana
Sign Up to like & getrecommendations! Published in 2022 at "Frontiers in Physiology"
DOI: 10.3389/fphys.2022.938688
Abstract: The Anaphase Promoting Complex (APC/C), a large cullin-RING E3-type ubiquitin ligase, constitutes the ultimate target of the Spindle Assembly Checkpoint (SAC), an intricate regulatory circuit that ensures the high fidelity of chromosome segregation in eukaryotic… read more here.
Keywords: ubiquitin ligase; biochemical biophysical; arabidopsis thaliana; cdc20 ... See more keywords
Biochemical and Biophysical Characterisation of the Hepatitis E Virus Guanine-7-Methyltransferase
Sign Up to like & getrecommendations! Published in 2022 at "Molecules"
DOI: 10.3390/molecules27051505
Abstract: Hepatitis E virus (HEV) is an understudied pathogen that causes infection through fecal contaminated drinking water and is prominently found in South Asian countries. The virus affects ~20 million people annually, leading to ~60,000 infections… read more here.
Keywords: biochemical biophysical; hepatitis virus; biophysical characterisation; mtase ... See more keywords