Foundations of identifying individual chromosomes by imaging flow cytometry with applications in radiation biodosimetry.
Sign Up to like & getrecommendations! Published in 2017 at "Methods"
DOI: 10.1016/j.ymeth.2016.08.006
Abstract: Biodosimetry is an important tool for triage in the case of large-scale radiological or nuclear emergencies, but traditional microscope-based methods can be tedious and prone to scorer fatigue. While the dicentric chromosome assay (DCA) has… read more here.
Keywords: imaging flow; flow cytometry; biodosimetry; individual chromosomes ... See more keywords
Development of the FAST-DOSE assay system for high-throughput biodosimetry and radiation triage
Sign Up to like & getrecommendations! Published in 2020 at "Scientific Reports"
DOI: 10.1038/s41598-020-69460-7
Abstract: Following a large-scale radiological incident, there is a need for FDA-approved biodosimetry devices and biomarkers with the ability to rapidly determine past radiation exposure with sufficient accuracy for early population triage and medical management. Towards… read more here.
Keywords: system; exposure; biodosimetry; fast dose ... See more keywords
Comparison of inexperienced operators and experts in γH2A.X and 53BP1 foci assay for high-throughput biodosimetry approaches in a mass casualty incident
Sign Up to like & getrecommendations! Published in 2020 at "International Journal of Radiation Biology"
DOI: 10.1080/09553002.2020.1793024
Abstract: Abstract Purpose In case of population exposure by ionizing radiation, a fast and reliable dose assessment of exposed and non-exposed individuals is crucial important. In initial triage, physicians have to take fast decisions whom to… read more here.
Keywords: foci assay; h2a 53bp1; foci scoring; biodosimetry ... See more keywords
Radiation-induced unrepairable DSBs: their role in the late effects of radiation and possible applications to biodosimetry
Sign Up to like & getrecommendations! Published in 2018 at "Journal of Radiation Research"
DOI: 10.1093/jrr/rrx074
Abstract: Abstract Although the vast majority of DNA damage induced by radiation exposure disappears rapidly, some lesions remain in the cell nucleus in very small quantities for days to months. These lesions may cause a considerable… read more here.
Keywords: late effects; unrepairable dsbs; effects radiation; biodosimetry ... See more keywords
BIODOSIMETRY AND BIODOSIMETRY NETWORKS FOR MANAGING RADIATION EMERGENCY
Sign Up to like & getrecommendations! Published in 2018 at "Radiation Protection Dosimetry"
DOI: 10.1093/rpd/ncy137
Abstract: Biological dosimetry enables individual dose reconstruction in the case of unclear or inconsistent radiation exposure situations, especially when a direct measurement of ionizing radiation is not or is no longer possible. To be prepared for… read more here.
Keywords: networks managing; biodosimetry networks; managing radiation; biodosimetry biodosimetry ... See more keywords
Radiation-induced gene expression changes used for biodosimetry and clinical outcome prediction: challenges and promises.
Sign Up to like & getrecommendations! Published in 2023 at "Cytogenetic and genome research"
DOI: 10.1159/000530947
Abstract: As the war in Ukraine progresses, the radiological and nuclear threat has never been as real as now. The formation of life-threatening acute radiation syndrome (ARS), in particular after the deployment of a nuclear weapon… read more here.
Keywords: ars severity; biodosimetry; clinical outcome; radiation ... See more keywords
Impact of inflammatory signaling on radiation biodosimetry: mouse model of inflammatory bowel disease
Sign Up to like & getrecommendations! Published in 2019 at "BMC Genomics"
DOI: 10.1186/s12864-019-5689-y
Abstract: BackgroundIonizing Radiation (IR) is a known pro-inflammatory agent and in the process of development of biomarkers for radiation biodosimetry, a chronic inflammatory disease condition could act as a confounding factor. Hence, it is important to… read more here.
Keywords: biodosimetry; mouse model; disease; radiation ... See more keywords
CytoRADx: A High-Throughput, Standardized Biodosimetry Diagnostic System Based on the Cytokinesis-Block Micronucleus Assay
Sign Up to like & getrecommendations! Published in 2021 at "Radiation Research"
DOI: 10.1667/rade-20-00030.1
Abstract: In a large-scale catastrophe, such as a nuclear detonation in a major city, it will be crucial to accurately diagnose large numbers of people to direct scarce medical resources to those in greatest need. Currently… read more here.
Keywords: system; radiation; cytokinesis block; biodosimetry ... See more keywords
Developing Human Radiation Biodosimetry Models: Testing Cross-Species Conversion Approaches Using an Ex Vivo Model System
Sign Up to like & getrecommendations! Published in 2017 at "Radiation Research"
DOI: 10.1667/rr14655.1
Abstract: In the event of a large-scale radiation exposure, accurate and quick assessment of radiation dose received would be critical for triage and medical treatment of large numbers of potentially exposed individuals. Current methods of biodosimetry,… read more here.
Keywords: cross species; biodosimetry models; model; biodosimetry ... See more keywords
Automated Triage Radiation Biodosimetry: Integrating Imaging Flow Cytometry with High-Throughput Robotics to Perform the Cytokinesis-Block Micronucleus Assay
Sign Up to like & getrecommendations! Published in 2019 at "Radiation Research"
DOI: 10.1667/rr15243.1
Abstract: The cytokinesis-block micronucleus (CBMN) assay has become a fully-validated and standardized method for radiation biodosimetry. The assay is typically performed using microscopy, which is labor intensive, time consuming and impractical after a large-scale radiological/nuclear event.… read more here.
Keywords: robotics; microscopy; radiation; biodosimetry ... See more keywords
Use of a Humanized Mouse Model System in the Validation of Human Radiation Biodosimetry Standards
Sign Up to like & getrecommendations! Published in 2019 at "Radiation Research"
DOI: 10.1667/rr15283.1
Abstract: After a planned or unplanned radiation exposure, determination of absorbed dose has great clinical importance, informing treatment and triage decisions in the exposed individuals. Biodosimetry approaches allow for determination of dose in the absence of… read more here.
Keywords: radiation; mouse model; radiation biodosimetry; biodosimetry ... See more keywords