BLI-MS: Combining biolayer interferometry and mass spectrometry.
Sign Up to like & getrecommendations! Published in 2021 at "Proteomics"
DOI: 10.1002/pmic.202100031
Abstract: Biolayer Interferometry (BLI) is a technology which allows to study the affinity between two interacting macro-molecules and to visualize their kinetic of interaction in real time. In this work we combine BLI interaction measurement with… read more here.
Keywords: biolayer interferometry; mass spectrometry;
The strengths and limitations of using biolayer interferometry to monitor equilibrium titrations of biomolecules
Sign Up to like & getrecommendations! Published in 2020 at "Protein Science"
DOI: 10.1002/pro.3827
Abstract: Every method used to quantify biomolecular interactions has its own strengths and limitations. To quantify protein‐DNA binding affinities, nitrocellulose filter binding assays with 32P‐labeled DNA quantify Kd values from 10−12 to 10−8 M but have… read more here.
Keywords: protein; equilibrium titrations; dna; biolayer interferometry ... See more keywords
Biolayer interferometry provides a robust method for detecting DNA binding small molecules in microbial extracts
Sign Up to like & getrecommendations! Published in 2020 at "Analytical and Bioanalytical Chemistry"
DOI: 10.1007/s00216-020-03079-5
Abstract: DNA replication is an exceptional point of therapeutic intervention for many cancer types and several small molecules targeting DNA have been developed into clinically used antitumor agents. Many of these molecules are naturally occurring metabolites… read more here.
Keywords: binding small; dna; small molecules; biolayer interferometry ... See more keywords
Biolayer Interferometry-based FcγRIIa binding assay for a therapeutic antibody with strong effector function.
Sign Up to like & getrecommendations! Published in 2020 at "Analytical biochemistry"
DOI: 10.1016/j.ab.2020.113842
Abstract: FcγRIIa receptor binding is part of the mechanism of action for many therapeutic antibodies. AlphaScreen® technology and Biolayer Interferometry (BLI) are often used to assess protein-protein interactions. Recently we demonstrated that the presence of aggregates… read more here.
Keywords: based riia; riia; binding assay; biolayer interferometry ... See more keywords
In vitro biolayer interferometry analysis of acetylcholinesterase as a potential target of aryl-organophosphorus flame-retardants.
Sign Up to like & getrecommendations! Published in 2020 at "Journal of hazardous materials"
DOI: 10.1016/j.jhazmat.2020.124999
Abstract: Organophosphorus flame retardants (OPFRs) have been implicated as neurotoxicants, but their potential neurotoxicity and mechanisms remain poorly understood. Herein, we investigated the neurotoxicity of selected OPFRs using zebrafish as a model organism. Environmentally relevant concentrations… read more here.
Keywords: ache; organophosphorus flame; biolayer interferometry; aryl opfrs ... See more keywords
Suppressing Nonspecific Binding in Biolayer Interferometry Experiments for Weak Ligand–Analyte Interactions
Sign Up to like & getrecommendations! Published in 2022 at "ACS Omega"
DOI: 10.1021/acsomega.1c05659
Abstract: Quantitative analysis of protein–protein interactions (PPIs) using biolayer interferometry (BLI) requires effective suppression of nonspecific binding (NSB) between analytes and biosensors. In particular, the study of weak interactions (i.e., KD > 1 μM) requires high… read more here.
Keywords: weak interactions; biolayer interferometry; new nsb; nonspecific binding ... See more keywords
Direct Detection of Low-Molecular-Weight Compounds in 2D and 3D Aptasensors by Biolayer Interferometry.
Sign Up to like & getrecommendations! Published in 2020 at "ACS sensors"
DOI: 10.1021/acssensors.0c00925
Abstract: The direct biolayer interferometry (BLI) measurement of low-molecular-weight (LMW) analytes ( read more here.
Keywords: low molecular; aptamer density; biolayer interferometry; molecular weight ... See more keywords
Use of Surface Plasmon Resonance and Biolayer Interferometry for the Study of Protein–Protein Interactions on the Example of an Enzyme of a Glycosyl Hydrolase Subtype (EC 3.2.1) and Specific Antibodies to It
Sign Up to like & getrecommendations! Published in 2017 at "Applied Biochemistry and Microbiology"
DOI: 10.1134/s0003683817070055
Abstract: Two previously obtained, full-size, fully human antibodies that reversibly bind the active form of an enzyme belonging to the subtype EC 3.2.1, which is used for substitutive enzymatic therapy in lysosomal storage diseases, have been… read more here.
Keywords: biolayer interferometry; resonance biolayer; surface plasmon; plasmon resonance ... See more keywords
Biolayer interferometry for DNA-protein interactions
Sign Up to like & getrecommendations! Published in 2022 at "PLoS ONE"
DOI: 10.1371/journal.pone.0263322
Abstract: Biolayer interferometry (BLI) is a widely utilized technique for determining macromolecular interaction dynamics in real time. Using changes in the interference pattern of white light reflected off a biosensor tip, BLI can determine binding parameters… read more here.
Keywords: dna; biolayer interferometry; dna protein; bli ... See more keywords
Evaluating the immunogenicity of heparin and heparin derivatives by measuring their binding to platelet factor 4 using biolayer interferometry
Sign Up to like & getrecommendations! Published in 2022 at "Frontiers in Molecular Biosciences"
DOI: 10.3389/fmolb.2022.966754
Abstract: Heparin (HP) is a polysaccharide that is widely used in the clinic as an anticoagulant. A major side effect associated with HP is the heparin-induced thrombocytopenia (HIT), which is initiated by the immune response to… read more here.
Keywords: heparin; immunogenicity; biolayer interferometry; platelet factor ... See more keywords
Biolayer Interferometry Analysis for a Higher Throughput Quantification of In-Process Samples of a Rotavirus Vaccine
Sign Up to like & getrecommendations! Published in 2022 at "Vaccines"
DOI: 10.3390/vaccines10101585
Abstract: Rotavirus A infection is a global leading cause of severe acute gastroenteritis associated with life-threatening diarrheal episodes in infants and young children. The disease burden is being reduced, namely due to a wider access to… read more here.
Keywords: quantification; biolayer interferometry; process; throughput ... See more keywords