Near-Infrared Bioluminescence Imaging of Animal Cells with Through-Bond Energy Transfer Cassette.
Sign Up to like & getrecommendations! Published in 2021 at "Methods in molecular biology"
DOI: 10.1007/978-1-0716-1258-3_10
Abstract: Coelenterazine (CTZ) is the most general substrate for marine luciferases. The present protocol introduces a near-infrared (NIR ) bioluminescence (BL) imaging of mammalian cells with a cyanine-5 (Cy5) dye-conjugated CTZ . This unique Cy5-conjugated CTZ,… read more here.
Keywords: ctz; bond energy; energy transfer; cy5 ctz ... See more keywords
Production of GFP and Luciferase-Expressing Reporter Macrophages for In Vivo Bioluminescence Imaging.
Sign Up to like & getrecommendations! Published in 2018 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-7860-1_8
Abstract: Macrophages have emerged as crucial regulators of tissue homeostasis, inflammation, and tissue regeneration. In vivo bioluminescence imaging could offer a powerful tool to study many poorly understood aspects of macrophage biology. Thus, we recently developed… read more here.
Keywords: reporter; vivo bioluminescence; reporter macrophages; bioluminescence imaging ... See more keywords
Real-Time Monitoring of Yersinia pestis Promoter Activity by Bioluminescence Imaging.
Sign Up to like & getrecommendations! Published in 2019 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-9541-7_7
Abstract: Bioluminescence imaging (BLI) has become a major strategy for real-time analysis of dynamic biological processes. In particular, bioluminescent reporter microorganisms have been designed to advance our understanding of infectious diseases. Non-invasive monitoring of light-emitting pathogenic… read more here.
Keywords: time monitoring; real time; bioluminescence imaging; time ... See more keywords
Self-illuminating NIR-II bioluminescence imaging probe based on silver sulfide quantum dots.
Sign Up to like & getrecommendations! Published in 2022 at "ACS nano"
DOI: 10.1021/acsnano.2c06667
Abstract: Bioluminescence (BL) imaging has emerged to tackle the potential challenges of fluorescence (FL) imaging including the autofluorescence background, inhomogeneous illumination over a wide imaging field, and the light-induced overheating effect. Taking advantage of the bioluminescence… read more here.
Keywords: quantum dots; self illuminating; silver sulfide; bioluminescence imaging ... See more keywords
Biolum' RGB: A Low-Cost, Versatile, and Sensitive Bioluminescence Imaging Instrument for a Broad Range of Users.
Sign Up to like & getrecommendations! Published in 2022 at "ACS sensors"
DOI: 10.1021/acssensors.2c00457
Abstract: Luminometer and imaging systems are used to detect and quantify low light produced by a broad range of bioluminescent proteins. Despite their everyday use in research, such instruments are costly and lack the flexibility to… read more here.
Keywords: imaging instrument; bioluminescence; low cost; biolum rgb ... See more keywords
Red-Shifted Coumarin Luciferins for Improved Bioluminescence Imaging.
Sign Up to like & getrecommendations! Published in 2023 at "Journal of the American Chemical Society"
DOI: 10.1021/jacs.2c07220
Abstract: Multicomponent bioluminescence imaging in vivo requires an expanded collection of tissue-penetrant probes. Toward this end, we generated a new class of near-infrared (NIR) emitting coumarin luciferin analogues (CouLuc-3s). The scaffolds were easily accessed from commercially… read more here.
Keywords: bioluminescence imaging; bioluminescence; shifted coumarin; red shifted ... See more keywords
Critical Considerations in Bioluminescence Imaging of Transplanted Islets
Sign Up to like & getrecommendations! Published in 2022 at "Pancreas"
DOI: 10.1097/mpa.0000000000002004
Abstract: Objectives In pancreatic islet transplantation studies, bioluminescence imaging enables quantitative and noninvasive tracking of graft survival. Amid the recent heightened interest in extrahepatic sites for islet and stem cell–derived beta-like cell transplantations, proper understanding the… read more here.
Keywords: considerations bioluminescence; bioluminescence; imaging transplanted; bioluminescence imaging ... See more keywords
Single-cell bioluminescence imaging of deep tissue in freely moving animals
Sign Up to like & getrecommendations! Published in 2018 at "Science"
DOI: 10.1126/science.aaq1067
Abstract: Improved spy tactics for single cells Bioluminescence imaging is a tremendous asset to medical research, providing a way to monitor living cells noninvasively within their natural environments. Advances in imaging methods allow researchers to measure… read more here.
Keywords: moving animals; bioluminescence imaging; bioluminescence; light source ... See more keywords
Neurobiological insights from bioluminescence imaging
Sign Up to like & getrecommendations! Published in 2017 at "Oncotarget"
DOI: 10.18632/oncotarget.20302
Abstract: Bioluminescence imaging has already been used for two decades to visualize cells and target genes completely non-invasively. Most frequently, firefly luciferase is applied which efficiently converts the substrate D-Luciferin in an enzymatic reaction that is… read more here.
Keywords: bioluminescence imaging; bioluminescence; brain; luciferase ... See more keywords
Bioluminescence Imaging of Matrix Metalloproteinases-2 and -9 Activities in Ethanol-injured Cornea of Mice
Sign Up to like & getrecommendations! Published in 2021 at "In Vivo"
DOI: 10.21873/invivo.12405
Abstract: Background/Aim: This study aimed to investigate the usefulness of in vivo bioluminescence imaging (BLI) to examine the role of matrix metalloproteinases (MMP)-2 and MMP-9 activation in the development and healing of ethanol-induced damage in the… read more here.
Keywords: cornea mice; eye; bioluminescence imaging; matrix metalloproteinases ... See more keywords
Establishment of a Novel Mouse Hepatocellular Carcinoma Model for Dynamic Monitoring of Tumor Development by Bioluminescence Imaging
Sign Up to like & getrecommendations! Published in 2022 at "Frontiers in Oncology"
DOI: 10.3389/fonc.2022.794101
Abstract: In this study, a novel mouse model of hepatocellular carcinoma (HCC) was established by simultaneously knocking out Pten and p53 suppressor genes and overexpressing c-Met and △90-β-catenin proto-oncogenes in the livers of mice via hydrodynamic… read more here.
Keywords: novel mouse; hepatocellular carcinoma; model; bioluminescence imaging ... See more keywords