An enzyme-mediated bioorthogonal labeling method for genome-wide mapping of 5-hydroxymethyluracil
Sign Up to like & getrecommendations! Published in 2021 at "Chemical Science"
DOI: 10.1039/d1sc03812e
Abstract: DNA 5-hydroxymethyluracil (5hmU) is a thymine modification existing in the genomes of various organisms. The post-replicative formation of 5hmU occurs via hydroxylation of thymine by ten-eleven translocation (TET) dioxygenases in mammals and J-binding proteins (JBPs)… read more here.
Keywords: enzyme mediated; bioorthogonal labeling; labeling method; mediated bioorthogonal ... See more keywords
Bioorthogonal labeling and profiling of N6-isopentenyladenosine (i6A) modified RNA
Sign Up to like & getrecommendations! Published in 2024 at "Nucleic Acids Research"
DOI: 10.1093/nar/gkae150
Abstract: Abstract Chemical modifications in RNAs play crucial roles in diversifying their structures and regulating numerous biochemical processes. Since the 1990s, several hydrophobic prenyl-modifications have been discovered in various RNAs. Prenyl groups serve as precursors for… read more here.
Keywords: bioorthogonal labeling; modified rna; isopentenyladenosine i6a; labeling profiling ... See more keywords