Articles with "biophysical characterization" as a keyword



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Biophysical characterization of Atg11, a scaffold protein essential for selective autophagy in yeast

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Published in 2018 at "FEBS Open Bio"

DOI: 10.1002/2211-5463.12355

Abstract: Autophagy is an intracellular degradation system in which the formation of an autophagosome is a key event. In budding yeast, autophagosomes are generated from the preautophagosomal structure (PAS), in which Atg11 and Atg17 function as… read more here.

Keywords: atg11 scaffold; biophysical characterization; scaffold protein; essential selective ... See more keywords
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Initial biophysical characterization of Amynthas gracilis giant extracellular hemoglobin (HbAg)

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Published in 2020 at "European Biophysics Journal"

DOI: 10.1007/s00249-020-01455-8

Abstract: The aim of the present work was the biophysical characterization of the Amynthas gracilis hemoglobin (HbAg). The oxy-HbAg optical absorption data, with Soret and Q bands centered at 415, 540 and 575 nm, were stable… read more here.

Keywords: hemoglobin hbag; hbag; biophysical characterization; oxy hbag ... See more keywords
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Biophysical characterization of Ca2+-binding of S100A5 and Ca2+-induced interaction with RAGE.

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Published in 2017 at "Biochemical and biophysical research communications"

DOI: 10.1016/j.bbrc.2016.12.143

Abstract: S100A5 is a calcium-binding protein of S100 family, which represents a major ligand to the receptor for advanced glycation end product (RAGE), a pattern recognition receptor engaged in diverse pathological processes. Here we have characterized… read more here.

Keywords: calcium binding; biophysical characterization; rage; ca2 ... See more keywords
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Biophysical Characterization of Membrane Pores Formed by Amyloid Beta(25-35)

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Published in 2017 at "Biophysical Journal"

DOI: 10.1016/j.bpj.2016.11.1245

Abstract: Amyloid β (Aβ) peptide contributes to Alzheimer's disease (AD) by a yet unidentified mechanism. In brain tissue, Aβ occurs in various forms, including a undecapeptide Aβ(25-35) (GSNKGAIIGLM), which exerts neurotoxic effect through mitochondrial dysfunction and/or… read more here.

Keywords: membrane pores; biophysical characterization; pores formed; membrane ... See more keywords
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Synthesis and Biophysical Characterization of the Chlorosulfolipids of Ochramonas danica

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Published in 2018 at "Biophysical Journal"

DOI: 10.1016/j.bpj.2017.11.129

Abstract: 85-Plat Synthesis and Biophysical Characterization of the Chlorosulfolipids of Ochramonas danica Grace M. McKenna, Frank R. Moss III, Matthew L. Landry, Noah Z. Burns, Steven G. Boxer. Chemistry, Stanford University, Stanford, CA, USA. The freshwater… read more here.

Keywords: biophysical characterization; ochramonas danica; danica; membrane ... See more keywords
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Biophysical characterization of intrinsically disordered human Golgi matrix protein GRASP65.

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Published in 2020 at "International journal of biological macromolecules"

DOI: 10.1016/j.ijbiomac.2020.08.126

Abstract: Golgi Reassembly and Stacking Proteins (GRASPs), including GRASP65/GRASP55, were firstly found as stacking factors of Golgi cisternae. Their involvement in other processes, such as unconventional protein secretion (UPS), have been demonstrated, suggesting GRASPs act as… read more here.

Keywords: protein; grasp65; biophysical characterization; golgi ... See more keywords
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Production and biophysical characterization of a mini-membrane protein, Ost4V23D: A functionally important mutant of yeast oligosaccharyltransferase subunit Ost4p.

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Published in 2017 at "Protein expression and purification"

DOI: 10.1016/j.pep.2017.07.009

Abstract: N-linked glycosylation of proteins is an essential and highly conserved co- and post-translational protein modification reaction that occurs in all eukaryotes. Oligosaccharyltransferase (OST), a multi-subunit membrane-associated enzyme complex, carries out this reaction. In the central… read more here.

Keywords: protein; biophysical characterization; functionally important; subunit ... See more keywords
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Structural and biophysical characterization of the major proteins from the seminal plasma of Dorper rams.

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Published in 2021 at "Theriogenology"

DOI: 10.1016/j.theriogenology.2021.02.019

Abstract: Pregnancy rates using frozen semen from rams are higher than for horses. One of the factors that positively influences this effect is the composition of low-molecular-weight proteins from seminal plasma, since the amounts of these… read more here.

Keywords: biophysical characterization; structural biophysical; plasma dorper; seminal plasma ... See more keywords
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Biophysical Characterization of Nucleolin Domains Crucial for Interaction with Telomeric and TERRA G-Quadruplexes.

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Published in 2023 at "Biochemistry"

DOI: 10.1021/acs.biochem.2c00641

Abstract: Nucleolin (NCL) is a well-characterized nucleic acid-binding protein; it binds to various canonical and noncanonical structures including single- and double-stranded DNA and RNA, hairpin, loops, and G-quadruplex structures. G-quadruplex structures are majorly formed in promoter,… read more here.

Keywords: characterization nucleolin; biophysical characterization; rna; nucleolin ... See more keywords
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Fighting the Huntington’s Disease with a G-Quadruplex-Forming Aptamer Specifically Binding to Mutant Huntingtin Protein: Biophysical Characterization, In Vitro and In Vivo Studies

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Published in 2022 at "International Journal of Molecular Sciences"

DOI: 10.3390/ijms23094804

Abstract: A set of guanine-rich aptamers able to preferentially recognize full-length huntingtin with an expanded polyglutamine tract has been recently identified, showing high efficacy in modulating the functions of the mutated protein in a variety of… read more here.

Keywords: huntington disease; huntingtin; biophysical characterization; aptamer ... See more keywords
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Biophysical Characterization and Cytocompatibility of Cellulose Cryogels Reinforced with Chitin Nanowhiskers

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Published in 2022 at "Polymers"

DOI: 10.3390/polym14132694

Abstract: Polysaccharide-based cryogels are promising materials for producing scaffolds in tissue engineering. In this work, we obtained ultralight (0.046–0.162 g/cm3) and highly porous (88.2–96.7%) cryogels with a complex hierarchical morphology by dissolving cellulose in phosphoric acid,… read more here.

Keywords: characterization cytocompatibility; biophysical characterization; cellulose cryogels; chitin nanowhiskers ... See more keywords