Effect of linkers on immobilization of scFvs with biotin–streptavidin interaction
Sign Up to like & getrecommendations! Published in 2018 at "Biotechnology and Applied Biochemistry"
DOI: 10.1002/bab.1645
Abstract: Single‐chain variable fragment antibodies (scFvs) are attractive for use in applications that require high specificity and binding to a target, such as biosensors. Previously, we demonstrated that a variety of scFvs can be immobilized onto… read more here.
Keywords: linkers immobilization; biotin; linker; effect linkers ... See more keywords
A one‐process production of completely biotinylated proteins in a T7 expression system
Sign Up to like & getrecommendations! Published in 2024 at "Biotechnology and Applied Biochemistry"
DOI: 10.1002/bab.2598
Abstract: Streptavidin is a tetrameric protein with high specificity and affinity for biotin. The interaction between avidin and biotin has become a valuable tool in nanotechnology. In recent years, the site‐specific biotin modification of proteins using… read more here.
Keywords: one process; biotinylated proteins; biotin; protein ... See more keywords
BioWF: A Naturally‐Fused, Di‐Domain Biocatalyst from Biotin Biosynthesis Displays an Unexpectedly Broad Substrate Scope
Sign Up to like & getrecommendations! Published in 2022 at "Chembiochem"
DOI: 10.1002/cbic.202200171
Abstract: The carbon backbone of biotin is constructed from the C7 di‐acid pimelate, which is converted to an acyl‐CoA thioester by an ATP‐dependent, pimeloyl‐CoA synthetase (PCAS, encoded by BioW). The acyl‐thioester is condensed with ʟ‐alanine in… read more here.
Keywords: fusion; biotin; domain; substrate ... See more keywords
Hydrogen‐Deuterium Exchange Defines Ligand‐Induced Conformational Changes to the Class III Biotin Protein Ligase from Saccharomyces cerevisiae
Sign Up to like & getrecommendations! Published in 2025 at "Chembiochem"
DOI: 10.1002/cbic.202500439
Abstract: Biotin protein ligase (BPL) catalyzes the covalent attachment of biotin onto biotin‐dependent enzymes, where it functions as an essential cofactor. Eukaryotic BPLs are distinct due to the presence of a large N‐terminal extension to the… read more here.
Keywords: saccharomyces cerevisiae; biotin; structure; biotin protein ... See more keywords
In Vivo Biotinylation of Antigens in E. coli.
Sign Up to like & getrecommendations! Published in 2017 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-6887-9_22
Abstract: Site-specific biotinylation of proteins is often the method of choice to enable efficient immobilization of a protein on a surface without interfering with protein folding. The tight interaction of biotin and streptavidin is frequently used… read more here.
Keywords: protein; biotin; biotinylation; vivo biotinylation ... See more keywords
Detection of biotin with zeptomole sensitivity using recombinant spores and a competition assay
Sign Up to like & getrecommendations! Published in 2020 at "Analytical and Bioanalytical Chemistry"
DOI: 10.1007/s00216-020-02854-8
Abstract: Detection of protein-binding analytes is important for many applications. Currently, various instrument-based techniques are used for detecting protein-binding analytes. However, such techniques have several limitations including high cost and time-consuming sample processing. In order to… read more here.
Keywords: recombinant spores; detection; biotin; using recombinant ... See more keywords
Specific and sensitive detection of Influenza A virus using a biotin-coated nanoparticle enhanced immunomagnetic assay
Sign Up to like & getrecommendations! Published in 2020 at "Analytical and Bioanalytical Chemistry"
DOI: 10.1007/s00216-020-03081-x
Abstract: This study reports the development of a sensitive magnetic bead-based enzyme-linked immunoassay (MELISA) for the pan-reactive detection of the Influenza A virus. The assay combines immunomagnetic beads and biotin-nanoparticle-based detection to quantify a highly conserved… read more here.
Keywords: detection; biotin; nanoparticle; virus ... See more keywords
Effect of biotin supplementation on milk yield of Girolando cows reared in a tropical climate
Sign Up to like & getrecommendations! Published in 2019 at "Tropical Animal Health and Production"
DOI: 10.1007/s11250-019-01862-w
Abstract: The objective of this study was to evaluate the effect of biotin supplementation on milk yield and the reproductive efficiency in Girolando cows. The study was conducted on a dairy farm located in central Brazil,… read more here.
Keywords: milk yield; biotin supplementation; girolando cows; biotin ... See more keywords
Rapid and sensitive determination of furaltadone metabolite 3-amino-5-morpholinomethyl-2-oxazolidinone by biotin–streptavidin-amplified time-resolved fluoroimmunoassay
Sign Up to like & getrecommendations! Published in 2018 at "Fisheries Science"
DOI: 10.1007/s12562-018-1211-8
Abstract: A competitive time-resolved fluoroimmunoassay (TRFIA) using a biotin–streptavidin system was developed for the detection and quantification of the furaltadone metabolite 3-amino-5-morpholinomethyl-2-oxazolidinone (AMOZ) in aquatic tissues. AMOZ-bovine serum albumin was coated to a solid phase and… read more here.
Keywords: resolved fluoroimmunoassay; biotin; biotin streptavidin; amoz ... See more keywords
The photostability of the commonly used biotin-4-fluorescein probe.
Sign Up to like & getrecommendations! Published in 2017 at "Analytical biochemistry"
DOI: 10.1016/j.ab.2017.05.019
Abstract: Biotin-4-fluorescein (B4F) is a commonly used fluorescent probe for studying biotin-(strept)avidin interactions. During a characterization study of an anti-biotin antibody, using B4F as the probe, we noticed a discrepancy in the expected and experimentally determined… read more here.
Keywords: biotin; probe; commonly used; biotin fluorescein ... See more keywords
Biotin oligonucleotide labeling reactions: A method to assess their effectiveness and reproducibility.
Sign Up to like & getrecommendations! Published in 2020 at "Analytical biochemistry"
DOI: 10.1016/j.ab.2020.113590
Abstract: The strong molecular interaction between biotin and streptavidin is widely used in the growing field of nucleic acid nanotechnology. Several biotin labeled oligonucleotide tools have been developed for the detection of biological molecules as well… read more here.
Keywords: effectiveness reproducibility; biotin; assess effectiveness; biotin oligonucleotide ... See more keywords