The photostability of the commonly used biotin-4-fluorescein probe.
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DOI: 10.1016/j.ab.2017.05.019
Abstract: Biotin-4-fluorescein (B4F) is a commonly used fluorescent probe for studying biotin-(strept)avidin interactions. During a characterization study of an anti-biotin antibody, using B4F as the probe, we noticed a discrepancy in the expected and experimentally determined… read more here.
Keywords: biotin; probe; commonly used; biotin fluorescein ... See more keywords