Biotin-tagged proteins: Reagents for efficient ELISA-based serodiagnosis and phage display-based affinity selection
Sign Up to like & getrecommendations! Published in 2018 at "PLoS ONE"
DOI: 10.1371/journal.pone.0191315
Abstract: The high-affinity interaction between biotin and streptavidin has opened avenues for using recombinant proteins with site-specific biotinylation to achieve efficient and directional immobilization. The site-specific biotinylation of proteins carrying a 15 amino acid long Biotin… read more here.
Keywords: biotin; immobilization; affinity; tag ... See more keywords
A simple method for non-denaturing purification of biotin-tagged proteins through competitive elution with free biotin.
Sign Up to like & getrecommendations! Published in 2019 at "BioTechniques"
DOI: 10.2144/btn-2019-0088
Abstract: The use of avidin or streptavidin in the purification of biotinylated proteins has been highly restricted due to the harsh and denaturing elution conditions. Here, we use biotinylated bovine serum albumin as a working model… read more here.
Keywords: elution; biotin; non denaturing; biotin tagged ... See more keywords
Liquid-Phase and Ultrahigh-Frequency-Acoustofluidics-Based Solid-Phase Synthesis of Biotin-Tagged 6′/3′-Sialyl-N-Acetylglucosamine by Sequential One-Pot Multienzyme System
Sign Up to like & getrecommendations! Published in 2020 at "Catalysts"
DOI: 10.3390/catal10111347
Abstract: 6′/3′-Sialylated N-acetyllactosamine (6′/3′-SLN) is important for discrimination of the source (human or avian) of influenza virus strains. Biotinylated oligosaccharides have been widely used for analysis and quick detection. The development of efficient strategies to synthesize… read more here.
Keywords: phase; solid phase; frequency acoustofluidics; ultrahigh frequency ... See more keywords