In Vivo Biotinylation of Antigens in E. coli.
Sign Up to like & getrecommendations! Published in 2017 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-6887-9_22
Abstract: Site-specific biotinylation of proteins is often the method of choice to enable efficient immobilization of a protein on a surface without interfering with protein folding. The tight interaction of biotin and streptavidin is frequently used… read more here.
Keywords: protein; biotin; biotinylation; vivo biotinylation ... See more keywords
Studying Purinoceptor Cell-Surface Expression by Protein Biotinylation.
Sign Up to like & getrecommendations! Published in 2020 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-9717-6_9
Abstract: Covalent labeling of protein with biotin (biotinylation) is a versatile technique which enables the capture and analysis of labeled protein with streptavidin-coated beads. It is particularly useful for detecting and quantifying the cell-surface expression of… read more here.
Keywords: protein; surface expression; biotinylation; cell surface ... See more keywords
Proximity Biotinylation as a Method for Mapping Proteins Associated with mtDNA in Living Cells.
Sign Up to like & getrecommendations! Published in 2017 at "Cell chemical biology"
DOI: 10.1016/j.chembiol.2017.02.002
Abstract: A recurring challenge in cell biology is to define the molecular components of macromolecular complexes of interest. The predominant method, immunoprecipitation, recovers only strong interaction partners that survive cell lysis and repeated detergent washes. We explored… read more here.
Keywords: mapping; biotinylation; proximity biotinylation; method ... See more keywords
Proximal Biotinylation Based Combinatory Approach for Isolating Integral Plasma Membrane Proteins.
Sign Up to like & getrecommendations! Published in 2020 at "Journal of proteome research"
DOI: 10.1021/acs.jproteome.0c00113
Abstract: Comprehensive profiling of the cell surface proteome has been challenging due to the lack of tools for an effective and reproducible way to isolate plasma membrane proteins from mammalian cells. Here, we employ a proximity-dependent… read more here.
Keywords: membrane proteins; biotinylation; approach; membrane ... See more keywords
DNA Modifications Enabling Proximity Biotinylation
Sign Up to like & getrecommendations! Published in 2022 at "Bioconjugate Chemistry"
DOI: 10.1101/2022.10.02.510503
Abstract: Advances in peroxidase- and biotin ligase-mediated signal amplification have enabled high-resolution subcellular mapping of endogenous RNA localization and protein-protein interactions. Application of these technologies has been limited to RNA and proteins because of the reactive… read more here.
Keywords: dna modifications; modifications enabling; proximity biotinylation; biotinylation ... See more keywords
Biotinylation as a tool to enhance the uptake of small molecules in Gram-negative bacteria
Sign Up to like & getrecommendations! Published in 2021 at "PLoS ONE"
DOI: 10.1371/journal.pone.0260023
Abstract: Antibiotic resistance is a major public health concern. The shrinking selection of effective antibiotics and lack of new development is making the situation worse. Gram-negative bacteria more specifically pose serious threat because of their double… read more here.
Keywords: accumulation; biotinylation; gram negative; enhanced accumulation ... See more keywords
Optical Sensors and Actuators for Probing Proximity-Dependent Biotinylation in Living Cells
Sign Up to like & getrecommendations! Published in 2022 at "Frontiers in Cellular Neuroscience"
DOI: 10.3389/fncel.2022.801644
Abstract: Proximity-dependent biotinylation techniques have been gaining wide applications in the systematic analysis of protein-protein interactions (PPIs) on a proteome-wide scale in living cells. The engineered biotin ligase TurboID is among the most widely adopted given… read more here.
Keywords: biotinylation; proximity dependent; dependent biotinylation; biotinylation living ... See more keywords
Generation of Peptides for Highly Efficient Proximity Utilizing Site-Specific Biotinylation in Cells
Sign Up to like & getrecommendations! Published in 2022 at "Life"
DOI: 10.3390/life12020300
Abstract: Protein tags are peptide sequences genetically embedded into a recombinant protein for various purposes, such as affinity purification, Western blotting, and immunofluorescence. Another recent application of peptide tags is in vivo labeling and analysis of… read more here.
Keywords: site specific; proximity utilizing; protein; biotinylation ... See more keywords