In Vivo Biotinylation of Antigens in E. coli.
Sign Up to like & getrecommendations! Published in 2017 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-6887-9_22
Abstract: Site-specific biotinylation of proteins is often the method of choice to enable efficient immobilization of a protein on a surface without interfering with protein folding. The tight interaction of biotin and streptavidin is frequently used… read more here.
Keywords: protein; biotin; biotinylation; vivo biotinylation ... See more keywords
Studying Purinoceptor Cell-Surface Expression by Protein Biotinylation.
Sign Up to like & getrecommendations! Published in 2020 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-9717-6_9
Abstract: Covalent labeling of protein with biotin (biotinylation) is a versatile technique which enables the capture and analysis of labeled protein with streptavidin-coated beads. It is particularly useful for detecting and quantifying the cell-surface expression of… read more here.
Keywords: protein; surface expression; biotinylation; cell surface ... See more keywords
Proximity Biotinylation as a Method for Mapping Proteins Associated with mtDNA in Living Cells.
Sign Up to like & getrecommendations! Published in 2017 at "Cell chemical biology"
DOI: 10.1016/j.chembiol.2017.02.002
Abstract: A recurring challenge in cell biology is to define the molecular components of macromolecular complexes of interest. The predominant method, immunoprecipitation, recovers only strong interaction partners that survive cell lysis and repeated detergent washes. We explored… read more here.
Keywords: mapping; biotinylation; proximity biotinylation; method ... See more keywords
Proximal Biotinylation Based Combinatory Approach for Isolating Integral Plasma Membrane Proteins.
Sign Up to like & getrecommendations! Published in 2020 at "Journal of proteome research"
DOI: 10.1021/acs.jproteome.0c00113
Abstract: Comprehensive profiling of the cell surface proteome has been challenging due to the lack of tools for an effective and reproducible way to isolate plasma membrane proteins from mammalian cells. Here, we employ a proximity-dependent… read more here.
Keywords: membrane proteins; biotinylation; approach; membrane ... See more keywords
Profiling the Cellular Surfaceome by Furan-Based Protein Biotinylation
Sign Up to like & getrecommendations! Published in 2025 at "Journal of Proteome Research"
DOI: 10.1021/acs.jproteome.5c00597
Abstract: The cellular surfaceome is crucial for cellular homeostasis and communication, with surface proteins acting as receptors, transporters, and enzymes. However, comprehensive analysis of the surfaceome is challenging due to the hydrophobic nature and low abundance… read more here.
Keywords: profiling cellular; cellular surfaceome; surfaceome; biotinylation ... See more keywords
DNA Modifications Enabling Proximity Biotinylation
Sign Up to like & getrecommendations! Published in 2022 at "Bioconjugate Chemistry"
DOI: 10.1101/2022.10.02.510503
Abstract: Advances in peroxidase- and biotin ligase-mediated signal amplification have enabled high-resolution subcellular mapping of endogenous RNA localization and protein-protein interactions. Application of these technologies has been limited to RNA and proteins because of the reactive… read more here.
Keywords: dna modifications; modifications enabling; proximity biotinylation; biotinylation ... See more keywords
Optimization of biotinylation protocol for next generation studies of red blood cell survival after transfusion
Sign Up to like & getrecommendations! Published in 2025 at "Transfusion"
DOI: 10.1111/trf.18286
Abstract: Red blood cell (RBC) biotinylation has increasingly become the preferred method for quantifying RBC post‐transfusion recovery (PTR). This study evaluated the feasibility of transfusing autologous RBCs labeled with biotin (BioRBCs) 48 h prior to transfusion. The… read more here.
Keywords: biotinylation; red blood; transfusion; blood cell ... See more keywords
Biotinylation as a tool to enhance the uptake of small molecules in Gram-negative bacteria
Sign Up to like & getrecommendations! Published in 2021 at "PLoS ONE"
DOI: 10.1371/journal.pone.0260023
Abstract: Antibiotic resistance is a major public health concern. The shrinking selection of effective antibiotics and lack of new development is making the situation worse. Gram-negative bacteria more specifically pose serious threat because of their double… read more here.
Keywords: accumulation; biotinylation; gram negative; enhanced accumulation ... See more keywords
AgrC biotinylation inhibits Staphylococcus aureus infection
Sign Up to like & getrecommendations! Published in 2025 at "PLOS One"
DOI: 10.1371/journal.pone.0318695
Abstract: Staphylococcus aureus (S. aureus) is a leading cause of nosocomial infections, particularly among antibiotic-resistant strains. S. aureus virulence is governed by the accessory gene regulator (Agr) quorum sensing (QS) system, which relies on AgrC, a… read more here.
Keywords: aureus; biotinylated aureus; agrc biotinylation; staphylococcus aureus ... See more keywords
Optical Sensors and Actuators for Probing Proximity-Dependent Biotinylation in Living Cells
Sign Up to like & getrecommendations! Published in 2022 at "Frontiers in Cellular Neuroscience"
DOI: 10.3389/fncel.2022.801644
Abstract: Proximity-dependent biotinylation techniques have been gaining wide applications in the systematic analysis of protein-protein interactions (PPIs) on a proteome-wide scale in living cells. The engineered biotin ligase TurboID is among the most widely adopted given… read more here.
Keywords: biotinylation; proximity dependent; dependent biotinylation; biotinylation living ... See more keywords
Biotinylation Interferes with Protein Ubiquitylation and Turnover in Arabidopsis—A Cautionary Insight for Proximity Labeling in Ubiquitylation Proteome Studies
Sign Up to like & getrecommendations! Published in 2025 at "International Journal of Molecular Sciences"
DOI: 10.3390/ijms26178248
Abstract: Nearly all eukaryotic proteins are turned over by the ubiquitin (Ub)-26S proteasome system (UPS). Despite its broad cellular roles, only a handful of UPS members, particularly the Ub E3 ligases that specifically recognize a protein… read more here.
Keywords: arabidopsis; ubiquitylation; protein ubiquitylation; proximity labeling ... See more keywords