Articles with "bisulfite" as a keyword



Whole-Genome Bisulfite Sequencing Using the OvationĀ® Ultralow Methyl-Seq Protocol.

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Published in 2018 at "Methods in molecular biology"

DOI: 10.1007/978-1-4939-7481-8_5

Abstract: The analysis of genome-wide epigenomic alterations including DNA methylation has become a subject of intensive research for many complex diseases. Whole-genome bisulfite sequencing (WGBS) using next-generation sequencing technologies can be considered the gold standard for… read more here.

Keywords: genome bisulfite; bisulfite; whole genome; bisulfite sequencing ... See more keywords

Whole-Genome Bisulfite Sequencing and Epigenetic Variation in Cereal Methylomes.

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Published in 2020 at "Methods in molecular biology"

DOI: 10.1007/978-1-4939-9865-4_10

Abstract: Whole-genome bisulfite sequencing (WGBS) is a technique used for the analysis of genome-wide DNA methylation patterns (DNA methylomes) at a single-base resolution. Here, I describe a simple DNA extraction method from rice endosperm and the… read more here.

Keywords: genome bisulfite; bisulfite; whole genome; bisulfite sequencing ... See more keywords

A fluorescent probe for colorimetric detection of bisulfite and application in sugar and red wine

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Published in 2019 at "Food Science and Biotechnology"

DOI: 10.1007/s10068-019-00571-2

Abstract: A new fluorescent probe made from (E)-2-(benzo[d]thiazol-2-yl)-3-(6-hydroxynaphthalen-2-yl) acrylonitrile (Probe 1) was synthesized for the determination of bisulfite concentrations in real food samples (red wine and sugar). Adding bisulfite to a Probe 1 solution caused a… read more here.

Keywords: fluorescent probe; bisulfite; probe; sugar ... See more keywords

Co3O4/CoO ceramic catalyst: Bisulfite assisted catalytic degradation of methylene blue

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Published in 2021 at "Ceramics International"

DOI: 10.1016/j.ceramint.2021.06.186

Abstract: Abstract Here in, a system of Co3O4/CoO catalyst activated with the aid of bisulfite (NaHSO3) was established for the decomposition of methylene blue (MB). The Co3O4/CoO material was designed by a two-step uniform precipitation and… read more here.

Keywords: catalyst; spectroscopy; co3o4 coo; bisulfite ... See more keywords

A novel colorimetric and ratiometric fluorescent probe for selective detection of bisulfite in real samples and living cells

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Published in 2019 at "Dyes and Pigments"

DOI: 10.1016/j.dyepig.2018.12.009

Abstract: Abstract An abnormal level of bisulfite can induce toxicological effects associated with lung cancer, cardiovascular and neurological disorders. Therefore, it is of significance to develop an effective fluorescent probe to detect bisulfite in living cells.… read more here.

Keywords: fluorescent probe; living cells; colorimetric ratiometric; bisulfite ... See more keywords

BiSulfite Bolt: A bisulfite sequencing analysis platform

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Published in 2021 at "GigaScience"

DOI: 10.1093/gigascience/giab033

Abstract: Abstract Background Bisulfite sequencing is commonly used to measure DNA methylation. Processing bisulfite sequencing data is often challenging owing to the computational demands of mapping a low-complexity, asymmetrical library and the lack of a unified… read more here.

Keywords: methylation; bisulfite; bsbolt; bisulfite sequencing ... See more keywords

Abstract 5050: Comparative analysis of somatic copy number variations and allelic imbalance in whole-genome bisulfite sequencing: challenges and insights

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Published in 2025 at "Cancer Research"

DOI: 10.1158/1538-7445.am2025-5050

Abstract: To assess the feasibility, reliability, and limitations of using Whole Genome Bisulfite Sequencing (WGBS) for detecting somatic copy number variation (SCNV) and tumor-specific allelic imbalance (AI). Background: In cancer genomics, the use of WGBS for… read more here.

Keywords: detection; number; cancer; whole genome ... See more keywords

Comprehensive comparison of enzymatic and bisulfite DNA methylation analysis in clinically relevant samples

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Published in 2025 at "Clinical Epigenetics"

DOI: 10.1186/s13148-025-01959-0

Abstract: Bisulfite conversion is considered the gold standard for DNA methylation analysis, but it damages DNA and performs sub-optimally with clinical samples (e.g., formalin-fixed paraffin-embedded and circulating free plasma DNA (cfDNA)). Here we describe a comprehensive… read more here.

Keywords: clinically relevant; methylation; dna methylation; conversion ... See more keywords

Stabilizers of edaravone aqueous solution and their action mechanisms. 1. Sodium bisulfite

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Published in 2017 at "Journal of Clinical Biochemistry and Nutrition"

DOI: 10.3164/jcbn.17-61

Abstract: Edaravone (3-methyl-1-phenyl-2-pyrazolin-5-one) has been used as a free radical scavenging drug for the treatment of acute ischemic stroke in Japan since 2001. Edaravone is given to patients intravenously; therefore, it is distributed in the form… read more here.

Keywords: sodium bisulfite; bisulfite; formation; solution ... See more keywords