Quantification of DNA methylation independent of sodium bisulfite conversion using methylation‐sensitive restriction enzymes and digital PCR
Sign Up to like & getrecommendations! Published in 2020 at "Human Mutation"
DOI: 10.1002/humu.24111
Abstract: Epigenetic regulation is important in human health and disease, but the exact mechanisms remain largely enigmatic. DNA methylation represents one epigenetic aspect but is challenging to quantify. In this study, we introduce a digital approach… read more here.
Keywords: bisulfite conversion; quantification; methylation; dna methylation ... See more keywords
Partial bisulfite conversion for unique template sequencing
Sign Up to like & getrecommendations! Published in 2018 at "Nucleic Acids Research"
DOI: 10.1093/nar/gkx1054
Abstract: Abstract We introduce a new protocol, mutational sequencing or muSeq, which uses sodium bisulfite to randomly deaminate unmethylated cytosines at a fixed and tunable rate. The muSeq protocol marks each initial template molecule with a… read more here.
Keywords: partial bisulfite; conversion unique; template; conversion ... See more keywords
Novel method utilizing bisulfite conversion with dual amplification‐refractory mutation system polymerase chain reaction to detect circulating pancreatic β‐cell cfDNA
Sign Up to like & getrecommendations! Published in 2022 at "Journal of Diabetes Investigation"
DOI: 10.1111/jdi.13806
Abstract: Several research groups have reported methods for quantifying pancreatic beta cell (β‐cell) injury by measuring β‐cell‐specific CpG unmethylation of the insulin gene in circulation using digital droplet PCR or next‐generation sequencing. However, these methods have… read more here.
Keywords: cell; conversion dual; utilizing bisulfite; bisulfite conversion ... See more keywords
A modified bisulfite conversion method for the detection of DNA methylation.
Sign Up to like & getrecommendations! Published in 2017 at "Epigenomics"
DOI: 10.2217/epi-2016-0174
Abstract: AIM Our purpose is to improve the conventional procedures for bisulfite conversion used to detect 5-methylcytosine in DNA. METHODS Impacts of different bisulfite salts, bisulfite conversion temperature, antioxidants and denaturants on DNA conversion and degradation… read more here.
Keywords: dna; conversion; bisulfite conversion; conversion method ... See more keywords