High-Resolution Analysis of 5-Hydroxymethylcytosine by TET-Assisted Bisulfite Sequencing.
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DOI: 10.1007/978-1-0716-0876-0_25
Abstract: DNA cytosine modification is an important epigenetic mechanism that serves critical functions in a variety of biological processes in development and disease. 5-Methylcytosine (5mC) and 5-hydroxymethylcytosine (5hmC) are the two most common epigenetic marks found… read more here.
Keywords: hydroxymethylcytosine; bisulfite sequencing; tet assisted; tet ... See more keywords
Low-Input Whole-Genome Bisulfite Sequencing.
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DOI: 10.1007/978-1-0716-1597-3_20
Abstract: DNA methylation can regulate gene expression by modulating chromatin accessibility and transcription factor binding on promoter and enhancer regions. Whole-genome bisulfite sequencing (WGBS) represents the most informative and comprehensive analysis to profile the DNA methylation… read more here.
Keywords: genome bisulfite; whole genome; dna; bisulfite sequencing ... See more keywords
Microdroplet PCR for Highly Multiplexed Targeted Bisulfite Sequencing.
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DOI: 10.1007/978-1-4939-7481-8_17
Abstract: Many methods exist for examining CpG DNA methylation. However, many of these are qualitative, laborious to apply to a large number of genes simultaneously, or are not easy to target to specific regions of interest.… read more here.
Keywords: pcr highly; bisulfite sequencing; highly multiplexed; microdroplet pcr ... See more keywords
Targeted Bisulfite Sequencing Using the SeqCap Epi Enrichment System.
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DOI: 10.1007/978-1-4939-7481-8_20
Abstract: Cytosine methylation has been shown to have a role in a host of biological processes. In mammalian biology these include stem cell differentiation, embryonic development, genomic imprinting, inflammation, and silencing of transposable elements. Given the… read more here.
Keywords: bisulfite sequencing; cytosine methylation; seqcap epi; biology ... See more keywords
Whole-Genome Bisulfite Sequencing Using the Ovation® Ultralow Methyl-Seq Protocol.
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DOI: 10.1007/978-1-4939-7481-8_5
Abstract: The analysis of genome-wide epigenomic alterations including DNA methylation has become a subject of intensive research for many complex diseases. Whole-genome bisulfite sequencing (WGBS) using next-generation sequencing technologies can be considered the gold standard for… read more here.
Keywords: genome bisulfite; bisulfite; whole genome; bisulfite sequencing ... See more keywords
Locus-Specific DNA Methylation Analysis by Targeted Deep Bisulfite Sequencing.
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DOI: 10.1007/978-1-4939-7774-1_19
Abstract: DNA methylation, i.e., the methylation of cytosine at carbon atom C5, has an important role in the regulation of gene expression. The methylation status of each cytosine in a specific genomic region can be determined… read more here.
Keywords: methylation; deep bisulfite; targeted deep; bisulfite sequencing ... See more keywords
Bisulfite Sequencing of RNA for Transcriptome-Wide Detection of 5-Methylcytosine.
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DOI: 10.1007/978-1-4939-8808-2_1
Abstract: A powerful method to determine the methylation status of specific cytosine residues within RNA is bisulfite sequencing. In combination with high-throughput sequencing methods cytosine methylation can be determined at nucleotide resolution on a transcriptome-wide level.… read more here.
Keywords: sequencing rna; bisulfite sequencing; rna transcriptome; wide detection ... See more keywords
Whole-Genome Bisulfite Sequencing and Epigenetic Variation in Cereal Methylomes.
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DOI: 10.1007/978-1-4939-9865-4_10
Abstract: Whole-genome bisulfite sequencing (WGBS) is a technique used for the analysis of genome-wide DNA methylation patterns (DNA methylomes) at a single-base resolution. Here, I describe a simple DNA extraction method from rice endosperm and the… read more here.
Keywords: genome bisulfite; bisulfite; whole genome; bisulfite sequencing ... See more keywords
Targeted Bisulfite Sequencing for Biomarker Discovery.
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DOI: 10.1016/j.ymeth.2020.07.006
Abstract: Cytosine methylation is one of the best studied epigenetic modifications. In mammals, DNA methylation patterns vary among cells and is mainly found in the CpG context. DNA methylation is involved in important processes during development… read more here.
Keywords: methylation; biomarker discovery; bisulfite sequencing; targeted bisulfite ... See more keywords
Extended-representation bisulfite sequencing of gene regulatory elements in multiplexed samples and single cells.
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DOI: 10.1038/s41587-021-00910-x
Abstract: The biological roles of DNA methylation have been elucidated by profiling methods based on whole-genome or reduced-representation bisulfite sequencing, but these approaches do not efficiently survey the vast numbers of non-coding regulatory elements in mammalian… read more here.
Keywords: representation bisulfite; bisulfite sequencing; regulatory elements; extended representation ... See more keywords
A new approach to decode DNA methylome and genomic variants simultaneously from double strand bisulfite sequencing.
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DOI: 10.1093/bib/bbab201
Abstract: Genetic and epigenetic contributions to various diseases and biological processes have been well-recognized. However, simultaneous identification of single-nucleotide variants (SNVs) and DNA methylation levels from traditional bisulfite sequencing data is still challenging. Here, we develop… read more here.
Keywords: dna; bisulfite sequencing; strand bisulfite; strand ... See more keywords