A programmable CRISPR/Cas9-based phage defense system for Escherichia coli BL21(DE3)
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DOI: 10.1186/s12934-020-01393-2
Abstract: Escherichia coli BL21 is arguably the most popular host for industrial production of proteins, and industrial fermentations are often plagued by phage infections. The CRISPR/Cas system is guided by a gRNA to cleave a specific… read more here.
Keywords: system; defense; crispr cas9; phage ... See more keywords
Technical Data of Heterologous Expression and Purification of SARS-CoV-2 Proteases Using Escherichia coli System
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DOI: 10.3390/data6090099
Abstract: The SARS-CoV-2 coronavirus expresses two essential proteases: firstly, the 3Chymotrypsin-like protease (3CLpro) or main protease (Mpro), and secondly, the papain-like protease (PLpro), both of which are considered as viable drug targets for the inhibition of… read more here.
Keywords: coli; cov2; cov proteases; bl21 ... See more keywords
Crystal Structure of Allantoinase from Escherichia coli BL21: A Molecular Insight into a Role of the Active Site Loops in Catalysis
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DOI: 10.3390/molecules28020827
Abstract: Allantoinase (ALLase; EC 3.5.2.5) possesses a binuclear metal center in which two metal ions are bridged by a posttranslationally carbamylated lysine. ALLase acts as a key enzyme for the biogenesis and degradation of ureides by… read more here.
Keywords: structure; site loops; active site; bl21 ... See more keywords