Biosynthesis of the human milk oligosaccharide 3‐fucosyllactose in metabolically engineered Escherichia coli via the salvage pathway through increasing GTP synthesis and β‐galactosidase modification
Sign Up to like & getrecommendations! Published in 2019 at "Biotechnology and Bioengineering"
DOI: 10.1002/bit.27160
Abstract: 3‐Fucosyllactose (3‐FL) is one of the major fucosylated oligosaccharides in human milk. Along with 2′‐fucosyllactose (2′‐FL), it is known for its prebiotic, immunomodulator, neonatal brain development, and antimicrobial function. Whereas the biological production of 2′‐FL… read more here.
Keywords: milk; production; coli bl21; biosynthesis human ... See more keywords
Cloning, expression and characterization of a thermo-alkali-stable xylanase from Aspergillus oryzae LC1 in Escherichia coli BL21(DE3).
Sign Up to like & getrecommendations! Published in 2019 at "Protein expression and purification"
DOI: 10.1016/j.pep.2019.105551
Abstract: In the present investigation, cloning and overexpression of xylanase (XynF1), the main xylanase of A. oryzae LC1, was performed in prokaryotic system E. coli BL21(DE3) to produce recombinant xylanase with high titer of specific activity… read more here.
Keywords: oryzae lc1; xylanase; bl21 de3; coli bl21 ... See more keywords
Refolding, purification, and characterization of constitutive-active human-Smad8 produced as inclusion bodies in ClearColi® BL21 (DE3).
Sign Up to like & getrecommendations! Published in 2021 at "Protein expression and purification"
DOI: 10.1016/j.pep.2021.105878
Abstract: Smad8 is a transcriptional regulator that participates in the intracellular signaling pathway of the transforming growth factor-β (TGF-β) family. Full-length Smad8 is an inactive protein in the absence of ligand stimulation. The expression of a… read more here.
Keywords: inclusion bodies; bl21 de3; smad8; clearcoli bl21 ... See more keywords
Efficient Biosynthesis of Sialyllacto-N-tetraose a by a Metabolically Engineered Escherichia coli BL21(DE3) Strain.
Sign Up to like & getrecommendations! Published in 2025 at "Journal of agricultural and food chemistry"
DOI: 10.1021/acs.jafc.4c12332
Abstract: Recently, the construction of metabolically engineered strains for the microbial synthesis of human milk oligosaccharides (HMOs) has attracted increasing attention. However, fewer efforts were made in the in vivo biosynthesis of complex HMOs, especially sialylated… read more here.
Keywords: escherichia coli; engineered escherichia; bl21 de3; sialyllacto tetraose ... See more keywords
CRISPR-Based Construction of a BL21 (DE3)-Derived Variant Strain Library to Rapidly Improve Recombinant Protein Production.
Sign Up to like & getrecommendations! Published in 2021 at "ACS synthetic biology"
DOI: 10.1021/acssynbio.1c00463
Abstract: Escherichia coli BL21 (DE3) is the most widely used host for recombinant protein expression. However, not every protein can be highly expressed in BL21 (DE3), so individual optimization strategies are often required for different proteins,… read more here.
Keywords: expression; strain; production; bl21 de3 ... See more keywords
A unique metallothionein-engineered in Escherichia coli for biosorption of lead, zinc, and cadmium; absorption or adsorption?
Sign Up to like & getrecommendations! Published in 2017 at "Microbiology"
DOI: 10.1134/s0026261717010064
Abstract: The present study introduced and evaluated modification of E. coli BL21 (DE3) to improve its biosorption capacity by the transfer of the Corynebacterium glutamicum metallothionein gene (C.gMT). The C.gMT sequence was extracted and cloned in… read more here.
Keywords: absorption; coli; biosorption; bl21 de3 ... See more keywords
Metabolic engineering of Escherichia coli BL21 (DE3) for de novo production of l-DOPA from d-glucose
Sign Up to like & getrecommendations! Published in 2019 at "Microbial Cell Factories"
DOI: 10.1186/s12934-019-1122-0
Abstract: BackgroundProduction of l-tyrosine is gaining grounds as the market size of 3,4-dihydroxyphenyl-l-alanine (l-DOPA) is expected to increase due to increasing cases of Parkinson’s disease a neurodegenerative disease. Attempts to overproduce l-tyrosine for conversion to l-DOPA… read more here.
Keywords: dopa; bl21 de3; coli bl21; production ... See more keywords
Acetate production from corn stover hydrolysate using recombinant Escherichia coli BL21 (DE3) with an EP-bifido pathway
Sign Up to like & getrecommendations! Published in 2024 at "Microbial Cell Factories"
DOI: 10.1186/s12934-024-02575-y
Abstract: Acetate is an important chemical feedstock widely applied in the food, chemical and textile industries. It is now mainly produced from petrochemical materials through chemical processes. Conversion of lignocellulose biomass to acetate by biotechnological pathways… read more here.
Keywords: acetate production; bl21 de3; coli pff; coli bl21 ... See more keywords
Improvement of sabinene tolerance of Escherichia coli using adaptive laboratory evolution and omics technologies
Sign Up to like & getrecommendations! Published in 2020 at "Biotechnology for Biofuels"
DOI: 10.1186/s13068-020-01715-x
Abstract: Biosynthesis of sabinene, a bicyclic monoterpene, has been accomplished in engineered microorganisms by introducing heterologous pathways and using renewable sugar as a carbon source. However, the efficiency and titers of this method are limited by… read more here.
Keywords: tolerance; sabinene tolerance; sabinene; bl21 de3 ... See more keywords
Metabolic network reconstruction and phenome analysis of the industrial microbe, Escherichia coli BL21(DE3)
Sign Up to like & getrecommendations! Published in 2018 at "PLoS ONE"
DOI: 10.1371/journal.pone.0204375
Abstract: Escherichia coli BL21(DE3) is an industrial model microbe for the mass-production of bioproducts such as biofuels, biorefineries, and recombinant proteins. However, despite its important role in scientific research and biotechnological applications, a high-quality metabolic network… read more here.
Keywords: model; bl21 de3; coli bl21; metabolic network ... See more keywords
Disrupting ribulose-5-phosphate metabolic flux enhances riboflavin production in Escherichia coli BL21(DE3)
Sign Up to like & getrecommendations! Published in 2025 at "PLOS One"
DOI: 10.1371/journal.pone.0336576
Abstract: Riboflavin (vitamin B2) is an essential water-soluble vitamin. To increase its production in a previously engineered strain, R203, we employed metabolic engineering strategies to improve the supply of ribulose-5-phosphate, a key precursor. Disruption of the… read more here.
Keywords: escherichia coli; riboflavin production; ribulose phosphate; bl21 de3 ... See more keywords