The complex synaptic pathways onto a looming‐detector neuron revealed using serial block‐face scanning electron microscopy
Sign Up to like & getrecommendations! Published in 2021 at "Journal of Comparative Neurology"
DOI: 10.1002/cne.25227
Abstract: The ability of locusts to detect looming stimuli and avoid collisions or predators depends on a neuronal circuit in the locust's optic lobe. Although comprehensively studied for over three decades, there are still major questions… read more here.
Keywords: face scanning; neuron; microscopy; block face ... See more keywords
Three‐dimensional fine structure of fibroblasts and other mesodermally derived tissues in the dermis of adults of the Bahamas lancelet (Chordata, Cephalohordata), as seen by serial block‐face scanning electron microscopy
Sign Up to like & getrecommendations! Published in 2022 at "Journal of Morphology"
DOI: 10.1002/jmor.21502
Abstract: Tissues of adult cephalochordates include sparsely distributed fibroblasts. Previous work on these cells has left unsettled such questions as their developmental origin, range of functions, and even their overall shape. Here, we describe fibroblasts of… read more here.
Keywords: block face; microscopy; bahamas lancelet; face scanning ... See more keywords
Imaging Plant Cells by High-Pressure Freezing and Serial block-face scanning electron microscopy.
Sign Up to like & getrecommendations! Published in 2020 at "Methods in molecular biology"
DOI: 10.1007/978-1-0716-0767-1_7
Abstract: This chapter describes methods to enhanced contrast of plant material processed by high-pressure freezing and freeze substitution for improved visualization by serial block-face scanning electron microscopy (SBEM). The contrast enhancing steps are based on a… read more here.
Keywords: plant; microscopy; serial block; pressure freezing ... See more keywords
Podocyte penetration of the glomerular basement membrane to contact on the mesangial cell at the lesion of mesangial interposition in lupus nephritis: a three-dimensional analysis by serial block-face scanning electron microscopy
Sign Up to like & getrecommendations! Published in 2019 at "Clinical and Experimental Nephrology"
DOI: 10.1007/s10157-019-01701-0
Abstract: BackgroundThe interaction among the glomerular components plays an important role in the development of glomerular lesions; thus, investigation of the ultrastructural three-dimensional (3D) configuration of the human glomerular cells and extracellular matrix (ECM) is important… read more here.
Keywords: three dimensional; microscopy; face scanning; serial block ... See more keywords
Combining serial block face and focused ion beam scanning electron microscopy for 3D studies of rare events.
Sign Up to like & getrecommendations! Published in 2019 at "Methods in cell biology"
DOI: 10.1016/bs.mcb.2019.03.014
Abstract: Volume electron microscopy allows for the automated acquisition of serial-section imaging data that can be reconstructed in three-dimensions (3D) to provide a detailed, geometrically accurate view of cellular ultrastructure. Two, volume electron microscopy (EM) techniques,… read more here.
Keywords: electron microscopy; microscopy; scanning electron; serial block ... See more keywords
3D microstructure reconstruction of casting aluminum alloy based on serial block-face scanning electron microscopy
Sign Up to like & getrecommendations! Published in 2019 at "Journal of Alloys and Compounds"
DOI: 10.1016/j.jallcom.2018.11.188
Abstract: Abstract In this work, the inner microstructure of aluminum-silicon casting alloy was experimentally investigated to reveal the spatial morphology and phase distribution. Its industrial use is as a light-weight structural material. As a powerful three-dimensional… read more here.
Keywords: electron microscopy; microscopy; face scanning; scanning electron ... See more keywords
Determination of secretory granule maturation times in pancreatic islet β-cells by serial block face scanning electron microscopy.
Sign Up to like & getrecommendations! Published in 2020 at "Journal of structural biology"
DOI: 10.1016/j.jsb.2020.107584
Abstract: It is shown how serial block-face electron microscopy (SBEM) of insulin-secreting β-cells in wild-type mouse pancreatic islets of Langerhans can be used to determine maturation times of secretory granules. Although SBEM captures the β -cell… read more here.
Keywords: secretory; microscopy; electron microscopy; serial block ... See more keywords
A Semi-Automated Workflow for Segmenting Contents of Single Cardiac Cells from Serial-Block-Face Scanning Electron Microscopy Data
Sign Up to like & getrecommendations! Published in 2017 at "Microscopy and Microanalysis"
DOI: 10.1017/s143192761700188x
Abstract: Serial-block-face scanning electron microscopy (SBFSEM) has revolutionized electron microscopy of cellular ultrastructure by providing a high throughput method to image large fields of view at tens of nanometer (nm) resolution. These data are enabling scientists… read more here.
Keywords: microscopy; electron microscopy; face scanning; serial block ... See more keywords
Biological serial block face scanning electron microscopy at improved z-resolution based on Monte Carlo model
Sign Up to like & getrecommendations! Published in 2018 at "Scientific Reports"
DOI: 10.1038/s41598-018-31231-w
Abstract: Serial block-face electron microscopy (SBEM) provides nanoscale 3D ultrastructure of embedded and stained cells and tissues in volumes of up to 107 µm3. In SBEM, electrons with 1–3 keV energies are incident on a specimen block, from… read more here.
Keywords: resolution; electron; block face; microscopy ... See more keywords
Serial block‐face scanning electron microscopy of erythrocytes protruding through the human placental syncytiotrophoblast
Sign Up to like & getrecommendations! Published in 2017 at "Journal of Anatomy"
DOI: 10.1111/joa.12658
Abstract: The syncytiotrophoblast forms a continuous barrier between the maternal and fetal circulations. Here we present a serial block‐face scanning electron microscopy (SBFSEM) study, based on a single image stack, showing pooling of fetal blood underneath… read more here.
Keywords: microscopy; syncytiotrophoblast; face scanning; serial block ... See more keywords
Morphological process of podocyte development revealed by block-face scanning electron microscopy
Sign Up to like & getrecommendations! Published in 2017 at "Journal of Cell Science"
DOI: 10.1242/jcs.187815
Abstract: ABSTRACT Podocytes present a unique 3D architecture specialized for glomerular filtration. However, several 3D morphological aspects on podocyte development remain partially understood because they are difficult to reveal using conventional scanning electron microscopy (SEM). Here,… read more here.
Keywords: block face; development; foot processes; microscopy ... See more keywords