Development of PCR Blocking Primers Enabling DNA Metabarcoding Analysis of Dietary Composition in Hematophagous Sea Lamprey
Sign Up to like & getrecommendations! Published in 2025 at "Ecology and Evolution"
DOI: 10.1002/ece3.71999
Abstract: ABSTRACT Conventional dietary assessments are challenging in hematophagous species, particularly in sea lamprey ( Petromyzon marinus ). However, recent technological developments and molecular approaches have provided an attractive alternative through the use of DNA metabarcoding.… read more here.
Keywords: blocking primers; dna metabarcoding; sea lamprey; dna ... See more keywords
Unravel the hidden protistan diversity: application of blocking primers to suppress PCR amplification of metazoan DNA
Sign Up to like & getrecommendations! Published in 2017 at "Applied Microbiology and Biotechnology"
DOI: 10.1007/s00253-017-8565-1
Abstract: Planktonic protists, including both autotroph and heterotroph, have been recognized as a major contributor to primary production and consumers of bacteria, archaea and picophytoplankton. However, the understanding of protistan diversity is typically impeded by the… read more here.
Keywords: blocking primers; amplification metazoan; protistan diversity; diversity ... See more keywords