CRISPR/Cas9-based precision tagging of essential genes in bloodstream form African trypanosomes.
Sign Up to like & getrecommendations! Published in 2022 at "Molecular and biochemical parasitology"
DOI: 10.1016/j.molbiopara.2022.111476
Abstract: Proteins of interest are frequently expressed with a fusion-tag to facilitate experimental analysis. In trypanosomatids, which are typically diploid, a tag-encoding DNA fragment is typically fused to one native allele. However, since recombinant cells represent… read more here.
Keywords: bloodstream form; african trypanosomes; cas9 based; form african ... See more keywords
Diversity in new flagellum tip attachment in bloodstream form African trypanosomes
Sign Up to like & getrecommendations! Published in 2022 at "Molecular Microbiology"
DOI: 10.1111/mmi.14979
Abstract: The closely related parasites Trypanosoma brucei, T. congolense, and T. vivax cause neglected tropical diseases collectively known as African Trypanosomiasis. A characteristic feature of bloodstream form T. brucei is the flagellum that is laterally attached… read more here.
Keywords: tip attachment; bloodstream form; flagellum; new flagellum ... See more keywords
Subcellular protein localisation of Trypanosoma brucei bloodstream form-upregulated proteins maps stage-specific adaptations
Sign Up to like & getrecommendations! Published in 2022 at "Wellcome Open Research"
DOI: 10.12688/wellcomeopenres.18586.2
Abstract: Genome-wide subcellular protein localisation in Trypanosoma brucei, through our TrypTag project, has comprehensively dissected the molecular organisation of this important pathogen. Powerful as this resource is, T. brucei has multiple developmental forms and we previously… read more here.
Keywords: localisation; bloodstream form; stage specific;
Novel aspects of iron homeostasis in pathogenic bloodstream form Trypanosoma brucei
Sign Up to like & getrecommendations! Published in 2021 at "PLoS Pathogens"
DOI: 10.1371/journal.ppat.1009696
Abstract: Iron is an essential regulatory signal for virulence factors in many pathogens. Mammals and bloodstream form (BSF) Trypanosoma brucei obtain iron by receptor-mediated endocytosis of transferrin bound to receptors (TfR) but the mechanisms by which… read more here.
Keywords: iron homeostasis; brucei; trypanosoma brucei; bloodstream form ... See more keywords
Metabolic insights into phosphofructokinase inhibition in bloodstream-form trypanosomes
Sign Up to like & getrecommendations! Published in 2023 at "Frontiers in Cellular and Infection Microbiology"
DOI: 10.3389/fcimb.2023.1129791
Abstract: Previously, we reported the development of novel small molecules that are potent inhibitors of the glycolytic enzyme phosphofructokinase (PFK) of Trypanosoma brucei and related protists responsible for serious diseases in humans and domestic animals. Cultured… read more here.
Keywords: increase; bloodstream form; form; form trypanosomes ... See more keywords