Western blot normalization: Time to choose a proper loading control seriously
Sign Up to like & getrecommendations! Published in 2023 at "ELECTROPHORESIS"
DOI: 10.1002/elps.202200222
Abstract: Recent research has questioned the validity of housekeeping proteins in Western blot. Our present study proposed new ideas for Western blot normalization that improved the reproducibility of scientific research. We used the Gene Expression Omnibus… read more here.
Keywords: proper loading; loading control; blot normalization; western blot ... See more keywords
Western Blot as a Support Technique for Immunohistochemistry to Detect Programmed Cell Death Ligand 1 Expression.
Sign Up to like & getrecommendations! Published in 2021 at "Methods in molecular biology"
DOI: 10.1007/978-1-0716-1278-1_5
Abstract: Antibody selection and optimization are crucial to guarantee accurate and reproducible results when using such antibodies for applications such as western blot analysis and immunohistochemistry (IHC). This is especially important when selecting good candidate antibodies… read more here.
Keywords: technique; immunohistochemistry; western blot; programmed cell ... See more keywords
A new background subtraction method for Western blot densitometry band quantification through image analysis software.
Sign Up to like & getrecommendations! Published in 2018 at "Journal of immunological methods"
DOI: 10.1016/j.jim.2018.03.004
Abstract: Since its first description, Western blot has been widely used in molecular labs. It constitutes a multistep method that allows the detection and/or quantification of proteins from simple to complex protein mixtures. Western blot quantification… read more here.
Keywords: analysis; western blot; method; blot ... See more keywords
Blame it on Southern, but it's a western blot
Sign Up to like & getrecommendations! Published in 2017 at "Autophagy"
DOI: 10.1080/15548627.2016.1255382
Abstract: ABSTRACT Edwin M. Southern is a professor emeritus at the University of Oxford. He is perhaps best known for development of the “Southern blot” (Dr. Southern was at the University of Edinburgh when he wrote… read more here.
Keywords: southern western; western blot; blame southern; biology ... See more keywords
Performance of the HIV Blot 2.2, INNO-LIA HIV I/II Score, and Geenius HIV 1/2 Confirmatory Assay for use in HIV confirmation
Sign Up to like & getrecommendations! Published in 2018 at "PLoS ONE"
DOI: 10.1371/journal.pone.0199502
Abstract: In view of recent revised recommendations for human immunodeficiency virus (HIV) confirmatory testing, the performance of 3 HIV confirmatory assays was compared. Using the HIV Blot 2.2 (MP-WB), the INNO-LIA HIV I/II Score (INNO), and… read more here.
Keywords: hiv; geenius; performance; blot ... See more keywords
Erratum. Adipocyte Glucocorticoid Receptor Deficiency Attenuates Aging- and HFD-Induced Obesity and Impairs the Feeding-Fasting Transition. Diabetes 2017;66:272–286
Sign Up to like & getrecommendations! Published in 2017 at "Diabetes"
DOI: 10.2337/db18-er02a
Abstract: In Fig. 2C displaying AKT and phosphorylated AKT (pAKT) expression in epididymal white adipose tissue (eWAT) and liver, the AKT immunoblotting was not the corresponding blot to the pAKT. The authors are replacing this figure… read more here.
Keywords: erratum adipocyte; pakt; adipocyte glucocorticoid; blot ... See more keywords
Is the Novel Slot Blot a Useful Method for Quantification of Intracellular Advanced Glycation End-Products?
Sign Up to like & getrecommendations! Published in 2023 at "Metabolites"
DOI: 10.3390/metabo13040564
Abstract: Various types of advanced glycation end-products (AGEs) have been identified and studied. I have reported a novel slot blot analysis to quantify two types of AGEs, glyceraldehyde-derived AGEs, also called toxic AGEs (TAGE), and 1,5-anhydro-D-fructose… read more here.
Keywords: quantification; advanced glycation; slot blot; novel slot ... See more keywords