Articles with "bottom proteomics" as a keyword



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A practical guide to interpreting and generating bottom‐up proteomics data visualizations

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Published in 2022 at "PROTEOMICS"

DOI: 10.1002/pmic.202100103

Abstract: Mass‐spectrometry based bottom‐up proteomics is the main method to analyze proteomes comprehensively and the rapid evolution of instrumentation and data analysis has made the technology widely available. Data visualization is an integral part of the… read more here.

Keywords: proteomics data; data visualizations; practical guide; bottom proteomics ... See more keywords
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In Search of a Universal Method: A Comparative Survey of Bottom-Up Proteomics Sample Preparation Methods

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Published in 2022 at "Journal of Proteome Research"

DOI: 10.1021/acs.jproteome.2c00265

Abstract: Robust, efficient and reproducible protein extraction and sample processing is a key step for bottom-up proteomics analyses. While many sample preparation protocols for mass spectrometry have been described, selecting an appropriate method remains challenging, since… read more here.

Keywords: preparation methods; sample preparation; preparation; bottom proteomics ... See more keywords
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Reversed-Phase Liquid Chromatography of Peptides for Bottom-Up Proteomics: A Tutorial.

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Published in 2022 at "Journal of proteome research"

DOI: 10.1021/acs.jproteome.2c00407

Abstract: The performance of the current bottom-up liquid chromatography hyphenated with mass spectrometry (LC-MS) analyses has undoubtedly been fueled by spectacular progress in mass spectrometry. It is thus not surprising that the MS instrument attracts the… read more here.

Keywords: liquid chromatography; reversed phase; liquid; phase liquid ... See more keywords

PrIntMap-R: An Online Application for Intraprotein Intensity and Peptide Visualization from Bottom-Up Proteomics.

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Published in 2023 at "Journal of proteome research"

DOI: 10.1021/acs.jproteome.2c00606

Abstract: Bottom-up proteomics (BUP) produces rich data, but visualization and analysis are time-consuming and often require programming skills. Many tools analyze these data at the proteome-level, but fewer options exist for individual proteins. Sequence coverage maps… read more here.

Keywords: printmap online; visualization; online application; bottom proteomics ... See more keywords
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An improved nanoflow RPLC-CZE-MS/MS system with high peak capacity and sensitivity for nanogram bottom-up proteomics.

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Published in 2019 at "Journal of proteome research"

DOI: 10.1021/acs.jproteome.9b00545

Abstract: Novel mass spectrometry (MS)-based proteomic tools with extremely high sensitivity and high peak capacity are required for comprehensive characterization of protein molecules in mass-limited samples. We reported a nanoRPLC-CZE-MS/MS system for deep bottom-up proteomics of… read more here.

Keywords: cze system; system; cze; sensitivity ... See more keywords