Differential release of cell-signaling metabolites by male and female bovine embryos cultured in vitro.
Sign Up to like & getrecommendations! Published in 2018 at "Theriogenology"
DOI: 10.1016/j.theriogenology.2018.03.038
Abstract: Male and female early bovine embryos show dimorphic transcription that impacts metabolism. Individual release of metabolites was examined in a 24h single culture medium from Day-6 male and female morulae that developed to Day-7 expanded… read more here.
Keywords: embryos; male female; bovine embryos; culture medium ... See more keywords
Role of endoplasmic reticulum stress on developmental competency and cryo-tolerance in bovine embryos.
Sign Up to like & getrecommendations! Published in 2019 at "Theriogenology"
DOI: 10.1016/j.theriogenology.2019.09.042
Abstract: Endoplasmic reticulum (ER) stress, a dysfunction in protein folding capacity of the ER, is involved in many physiological responses including mammalian reproductive systems. Studies have shown that ER stress interferes with the developmental process of… read more here.
Keywords: stress; developmental competency; cryo tolerance; bovine embryos ... See more keywords
Nobiletin enhances the development and quality of bovine embryos in vitro during two key periods of embryonic genome activation
Sign Up to like & getrecommendations! Published in 2021 at "Scientific Reports"
DOI: 10.1038/s41598-021-91158-7
Abstract: In vitro culture can alter the development and quality of bovine embryos. Therefore, we aimed to evaluate whether nobiletin supplementation during EGA improves embryonic development and blastocyst quality and if it affects PI3K/AKT signaling pathway.… read more here.
Keywords: bovine; bovine embryos; quality bovine; development quality ... See more keywords
67 Optimizing a protocol for isolating extracellular vesicles from medium conditioned by bovine embryos in vitro
Sign Up to like & getrecommendations! Published in 2019 at "Reproduction, Fertility and Development"
DOI: 10.1071/rdv31n1ab67
Abstract: In the absence of the maternal tract, pre-implantation bovine embryos cultured in group are able to promote their own development in vitro by releasing autocrine embryotropins. Recently we have identified extracellular vesicles (EV) among these… read more here.
Keywords: medium; conditioned bovine; extracellular vesicles; isolation ... See more keywords
133 CRISPR/Cas9 gene editing of invivo-fertilized bovine embryos via endoscopic oviductal flushing and electroporation of zygotes
Sign Up to like & getrecommendations! Published in 2020 at "Reproduction, Fertility and Development"
DOI: 10.1071/rdv32n2ab133
Abstract: In recent years, CRISPR/Cas9 has been used to efficiently edit the genomes of embryos in many animal models. Due to smaller anatomy, lower costs, and multiple ovulations, it is relatively simple to derive large numbers… read more here.
Keywords: crispr cas9; bovine embryos; electroporation; gene ... See more keywords
89 Embryonic metabolism orchestrates epigenetic mechanisms: What can we anticipate from the first cleavages?
Sign Up to like & getrecommendations! Published in 2020 at "Reproduction, Fertility and Development"
DOI: 10.1071/rdv32n2ab89
Abstract: Intermediates of the energy metabolism (as acetyl Co-A) can donate their acetyl group to introduce acetylation in histones, establishing a relationship between metabolism and epigenetic control in somatic and embryonic stem cells. Embryos with different… read more here.
Keywords: embryonic metabolism; acetylation; first cleavages; metabolism ... See more keywords
149 Uptake of C18:0 from culture media during invitro culture decreases cryosurvival rates of bovine embryos
Sign Up to like & getrecommendations! Published in 2021 at "Reproduction, Fertility and Development"
DOI: 10.1071/rdv33n2ab149
Abstract: Cryosurvival of invitro-produced bovine embryos is lower than that of invivo-produced embryos, limiting their usability in the field. Previous work showed that the embryo’s lipid composition relates to its quality and cryosurvival. The present study… read more here.
Keywords: c18; invitro; cryosurvival; sof ... See more keywords
Interleukin-6 requires JAK to stimulate inner cell mass expansion in bovine embryos.
Sign Up to like & getrecommendations! Published in 2019 at "Reproduction"
DOI: 10.1530/rep-19-0286
Abstract: Supplementing interleukin-6 (IL6) to in vitro produced bovine embryos increases inner cell mass (ICM) cell numbers in blastocysts. A series of studies were completed to further dissect this effect. Treatment with IL6 increased ICM cell… read more here.
Keywords: cell mass; inner cell; icm cell; cell numbers ... See more keywords
Metabolism-driven post-translational modifications of H3K9 in early bovine embryos.
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DOI: 10.1530/rep-21-0134
Abstract: Metabolic and molecular profiles were reported as different for bovine embryos with distinct kinetics during the first cleavages. In this study, we used this same developmental model (fast vs slow) to determine if the relationship… read more here.
Keywords: translational modifications; metabolism; embryos; post translational ... See more keywords
Differentially methylated regions identified in bovine embryos are not observed in adulthood
Sign Up to like & getrecommendations! Published in 2023 at "Animal Reproduction"
DOI: 10.1590/1984-3143-ar2022-0076
Abstract: Abstract The establishment of epigenetic marks during the reprogramming window is susceptible to environmental influences, and stimuli during this critical stage can cause altered DNA methylation in offspring. In a previous study, we found that… read more here.
Keywords: methylation; adulthood; methylated regions; differentially methylated ... See more keywords
Lower quality bovine embryos may be successfully used for sex determination
Sign Up to like & getrecommendations! Published in 2018 at "Veterinarni Medicina"
DOI: 10.17221/1884-vetmed
Abstract: Abstr Act : The aim of this study was to evaluate the effect of sex determination procedures in Day 7 to Day 8 bovine embryos of various quality. For the purposes of comparison we used… read more here.
Keywords: quality; embryos; sex determination; bovine embryos ... See more keywords