A new, simple, automatic vitrification device: preliminary results with murine and bovine oocytes and embryos
Sign Up to like & getrecommendations! Published in 2018 at "Journal of Assisted Reproduction and Genetics"
DOI: 10.1007/s10815-018-1210-9
Abstract: PurposeThis paper reports the use of a novel automatic vitrification device (Sarah, Fertilesafe, Israel) for cryopreservation of oocytes and embryos.MethodsMice oocytes (n = 40) and embryos (8 cells, n = 35 and blastocysts, n = 165), bovine embryos (2PN, n = 35), and… read more here.
Keywords: vitrification device; oocytes embryos; vitrification; bovine oocytes ... See more keywords
Effect of supplementation of medium with Bauhinia forficata recombinant lectins on expression of oxidative stress genes during in vitro maturation of bovine oocytes.
Sign Up to like & getrecommendations! Published in 2021 at "Reproductive toxicology"
DOI: 10.1016/j.reprotox.2021.05.012
Abstract: The lectin of Bauhinia forficata (nBfL) is a protein able to bind reversibly to N-acetylgalactosamine, performing several functions and one of them is the antiproliferative activity in tumor cells, but its effects have not yet… read more here.
Keywords: forficata; expression; maturation; bovine oocytes ... See more keywords
Birth of healthy calves after intra-follicular transfer (IFOT) of slaughterhouse derived immature bovine oocytes.
Sign Up to like & getrecommendations! Published in 2017 at "Theriogenology"
DOI: 10.1016/j.theriogenology.2017.04.009
Abstract: To circumvent the negative impacts of in vitro culture on bovine embryos, we have recently established a new method, the so called intra-follicular oocyte transfer (IFOT), enabling in vivo fertilization and in vivo development of in vitro matured oocytes… read more here.
Keywords: immature bovine; bovine; slaughterhouse; blastocyst stage ... See more keywords
161 Cumulus Cell Luteinization is Enhanced During Aging of Bovine Oocytes Matured In Vitro
Sign Up to like & getrecommendations! Published in 2018 at "Reproduction, Fertility and Development"
DOI: 10.1071/rdv30n1ab161
Abstract: Maturing mammalian oocytes can prevent luteinization of cumulus cells (CC) both in vivo and in vitro by regulating CC steroidogenesis (Gilchrist et al. 2004 Anim. Reprod. Sci. 82-83, 431-446; Ramirez et al. 2016 Anim. Reprod.… read more here.
Keywords: cumulus; bovine; expression; aging bovine ... See more keywords
Melatonin protects against paraquat‐induced damage during in vitro maturation of bovine oocytes
Sign Up to like & getrecommendations! Published in 2019 at "Journal of Pineal Research"
DOI: 10.1111/jpi.12532
Abstract: Paraquat (PQ), a broad‐spectrum agricultural pesticide, causes cellular toxicity by increasing oxidative stress levels in various biological systems, including the reproductive system. PQ exposure causes embryotoxicity and reduces the developmental abilities of embryos. However, there… read more here.
Keywords: bovine; induced damage; toxic effects; maturation ... See more keywords
Evaluation of fertilization capability of frozen-thawed completely immotile spermatozoa collected from a white bengal tiger after interspecific ICSI with bovine oocytes
Sign Up to like & getrecommendations! Published in 2022 at "Animal Reproduction"
DOI: 10.1590/1984-3143-ar2022-0034
Abstract: Abstract The objective of this study was to evaluate the fertilization capability of White Bengal Tiger frozen-thawed completely immotile spermatozoa after interspecific intracytoplasmic sperm injection (ICSI) with bovine oocytes. The fertilization status of presumptive zygotes… read more here.
Keywords: fertilization; bengal tiger; white bengal; bovine oocytes ... See more keywords
Granulosa cell-derived extracellular vesicles mitigate the detrimental impact of thermal stress on bovine oocytes and embryos
Sign Up to like & getrecommendations! Published in 2023 at "Frontiers in Cell and Developmental Biology"
DOI: 10.3389/fcell.2023.1142629
Abstract: Climate change-induced global warming results in rises in body temperatures above normal physiological levels (hyperthermia) with negative impacts on reproductive function in dairy and beef animals. Extracellular vesicles (EVs), commonly described as nano-sized, lipid-enclosed complexes,… read more here.
Keywords: stress; extracellular vesicles; granulosa cell; bovine oocytes ... See more keywords
Effects of β-Nicotinamide Mononucleotide, Berberine, and Cordycepin on Lipid Droplet Content and Developmental Ability of Vitrified Bovine Oocytes
Sign Up to like & getrecommendations! Published in 2023 at "Antioxidants"
DOI: 10.3390/antiox12050991
Abstract: Oocyte vitrification is crucial for livestock reproduction, germplasm conservation, and human-assisted reproduction, but the overabundance of lipids is highly detrimental to oocyte development. It is necessary to reduce the lipid droplet content of oocytes before… read more here.
Keywords: droplet content; vitrified bovine; lipid droplet; bovine oocytes ... See more keywords
Melatonin Abrogates the Anti-Developmental Effect of the AKT Inhibitor SH6 in Bovine Oocytes and Embryos
Sign Up to like & getrecommendations! Published in 2019 at "International Journal of Molecular Sciences"
DOI: 10.3390/ijms20122956
Abstract: Melatonin, a nighttime-secreted antioxidant hormone produced by the pineal gland, and AKT, a serine/threonine-specific protein kinase, have been identified as regulators for several cellular processes essential for reproduction. The current study aimed to investigate the… read more here.
Keywords: akt; akt inhibitor; melatonin; bovine oocytes ... See more keywords
Improvement of Fertilization Capacity and Developmental Ability of Vitrified Bovine Oocytes by JUNO mRNA Microinjection and Cholesterol-Loaded Methyl-β-Cyclodextrin Treatment
Sign Up to like & getrecommendations! Published in 2022 at "International Journal of Molecular Sciences"
DOI: 10.3390/ijms24010590
Abstract: Vitrification of oocytes is crucial for embryo biotechnologies, germplasm cryopreservation of endangered and excellent female animals, and the fertility of humans. However, vitrification significantly impairs the fertilization ability of oocytes, which significantly limits its widely… read more here.
Keywords: ability; fertilization; bovine oocytes; bovine ... See more keywords
Effects of epigallocatechin-3-gallate on bovine oocytes matured in vitro
Sign Up to like & getrecommendations! Published in 2018 at "Asian-Australasian Journal of Animal Sciences"
DOI: 10.5713/ajas.17.0880
Abstract: Objective Epigallocatechin-3-gallate (EGCG) is a major ingredient of catechin polyphenols and is considered one of the most promising bioactive compounds in green tea because of its strong antioxidant properties. However, the protective role of EGCG… read more here.
Keywords: bovine; cumulus cell; epigallocatechin gallate; egcg ... See more keywords