MALDI Mass Spectrometry Imaging of Lipids on Free-Floating Brain Sections and Immunohistochemically Colocalized Markers of Neurodegeneration.
Sign Up to like & getrecommendations! Published in 2022 at "Methods in molecular biology"
DOI: 10.1007/978-1-0716-2030-4_16
Abstract: In mass spectrometry imaging (MSI), the essential steps in sample preparation include collection and storage. The most widely used preservation procedure for MSI consists in freezing samples and storing them at temperatures below -80 °C. On… read more here.
Keywords: floating brain; mass spectrometry; spectrometry imaging; brain sections ... See more keywords
Synthesis and Assessment of Novel Probes for Imaging Tau Pathology in Transgenic Mouse and Rat Models
Sign Up to like & getrecommendations! Published in 2021 at "ACS Chemical Neuroscience"
DOI: 10.1021/acschemneuro.0c00790
Abstract: Aggregated tau protein is a core pathology present in several neurodegenerative diseases. Therefore, the development and application of positron emission tomography (PET) imaging radiotracers that selectively bind to aggregated tau in fibril form is of… read more here.
Keywords: tau; brain sections; synthesis assessment; pathology ... See more keywords
Optical clearing potential of immersion-based agents applied to thick mouse brain sections
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DOI: 10.1371/journal.pone.0216064
Abstract: We have previously demonstrated that the use of a commercially-available immersion-based optical clearing agent (OCA) enables, within 3–6 hours, three-dimensional visualization of subsurface exogenous fluorescent and absorbing markers of vascular architecture and neurodegenerative disease in… read more here.
Keywords: immersion based; mouse brain; brain sections; optical clearing ... See more keywords
An Automated Approach to Improve the Quantification of Pericytes and Microglia in Whole Mouse Brain Sections
Sign Up to like & getrecommendations! Published in 2021 at "eNeuro"
DOI: 10.1523/eneuro.0177-21.2021
Abstract: Abstract Whole slide scanning technology has enabled the generation of high-resolution images from complete tissue sections. However, commonly used analysis software is often unable to handle the large data files produced. Here, we present a… read more here.
Keywords: brain sections; automated approach; improve quantification; approach improve ... See more keywords
Selective In Vitro and Ex Vivo Staining of Brain Neurofibrillary Tangles and Amyloid Plaques by Novel Ethylene Ethynylene-Based Optical Sensors
Sign Up to like & getrecommendations! Published in 2023 at "Biosensors"
DOI: 10.3390/bios13020151
Abstract: The identification of protein aggregates as biomarkers for neurodegeneration is an area of interest for disease diagnosis and treatment development. In this work, we present novel super luminescent conjugated polyelectrolyte molecules as ex vivo sensors… read more here.
Keywords: vitro vivo; neurofibrillary tangles; brain; brain sections ... See more keywords