BRET‐based assay to monitor EGFR transactivation by the AT1R reveals Gq/11 protein‐independent activation and AT1R‐EGFR complexes
Sign Up to like & getrecommendations! Published in 2018 at "Biochemical Pharmacology"
DOI: 10.1016/j.bcp.2018.10.017
Abstract: &NA; The type 1 angiotensin II (AngII) receptor (AT1R) transactivates the epidermal growth factor receptor (EGFR), which leads to pathological remodeling of heart, blood vessels and kidney. End‐point assays are used as surrogates of EGFR… read more here.
Keywords: egfr; at1r; at1r egfr; egfr transactivation ... See more keywords
Engineered BRET-Based Biologic Light Sources Enable Spatio-Temporal Control Over Diverse Optogenetic Systems.
Sign Up to like & getrecommendations! Published in 2019 at "ACS synthetic biology"
DOI: 10.1021/acssynbio.9b00277
Abstract: Light-inducible optogenetic systems offer precise spatiotemporal control over a myriad of biologic processes. Unfortunately, current systems are inherently limited by their dependence on external light sources for their activation. Further, the utility of Laser/LED-based illumination… read more here.
Keywords: optogenetic systems; control; light sources; bret based ... See more keywords
BRET-Based Biosensors to Measure Agonist Efficacies in Histamine H1 Receptor-Mediated G Protein Activation, Signaling and Interactions with GRKs and β-Arrestins
Sign Up to like & getrecommendations! Published in 2022 at "International Journal of Molecular Sciences"
DOI: 10.3390/ijms23063184
Abstract: The histamine H1 receptor (H1R) is a G protein-coupled receptor (GPCR) and plays a key role in allergic reactions upon activation by histamine which is locally released from mast cells and basophils. Consequently, H1R is… read more here.
Keywords: interactions grks; activation; bret based; histamine receptor ... See more keywords
Developments in FRET- and BRET-Based Biosensors
Sign Up to like & getrecommendations! Published in 2022 at "Micromachines"
DOI: 10.3390/mi13101789
Abstract: Resonance energy transfer technologies have achieved great success in the field of analysis. Particularly, fluorescence resonance energy transfer (FRET) and bioluminescence resonance energy transfer (BRET) provide strategies to design tools for sensing molecules and monitoring… read more here.
Keywords: based biosensors; developments fret; resonance energy; energy transfer ... See more keywords
BRET-based RAS biosensors that show a novel small molecule is an inhibitor of RAS-effector protein-protein interactions
Sign Up to like & getrecommendations! Published in 2018 at "eLife"
DOI: 10.7554/elife.37122
Abstract: The RAS family of proteins is amongst the most highly mutated in human cancers and has so far eluded drug therapy. Currently, much effort is being made to discover mutant RAS inhibitors and in vitro… read more here.
Keywords: ras effector; based ras; protein protein; bret based ... See more keywords