Protonation states of Asp residues in the human Nudix hydrolase MTH1 contribute to its broad substrate recognition.
Sign Up to like & getrecommendations! Published in 2023 at "FEBS letters"
DOI: 10.1002/1873-3468.14611
Abstract: Human MutT homolog 1 (MTH1), also known as Nudix type motif 1 (NUDT1), hydrolyzes 8-oxo-dGTP and 2-oxo-dATP with broad substrate recognition and has attracted attention in anti-cancer therapeutics. Previous studies on MTH1 have proposed that… read more here.
Keywords: recognition; mth1; protonation; broad substrate ... See more keywords
Characterisation of a Bacterial Galactokinase with High Activity and Broad Substrate Tolerance for Chemoenzymatic Synthesis of 6‐Aminogalactose‐1‐Phosphate and Analogues
Sign Up to like & getrecommendations! Published in 2018 at "ChemBioChem"
DOI: 10.1002/cbic.201700477
Abstract: Glycosyl phosphates are important intermediates in many metabolic pathways and are substrates for diverse carbohydrate‐active enzymes. Thus, there is a need to develop libraries of structurally similar analogues that can be used as selective chemical… read more here.
Keywords: broad substrate; galactokinase; bacterial galactokinase; aminogalactose phosphate ... See more keywords
The Structural Determinants Accounting for the Broad Substrate Specificity of the Quorum Quenching Lactonase GcL
Sign Up to like & getrecommendations! Published in 2019 at "ChemBioChem"
DOI: 10.1002/cbic.201900024
Abstract: Quorum quenching lactonases are enzymes capable of hydrolyzing lactones, including N‐acyl homoserine lactones (AHLs). AHLs are molecules known as signals in bacterial communication dubbed quorum sensing. Bacterial signal disruption by lactonases was previously reported to… read more here.
Keywords: broad substrate; lactonase; quorum quenching; substrate specificity ... See more keywords
BioWF: A Naturally‐Fused, Di‐Domain Biocatalyst from Biotin Biosynthesis Displays an Unexpectedly Broad Substrate Scope
Sign Up to like & getrecommendations! Published in 2022 at "Chembiochem"
DOI: 10.1002/cbic.202200171
Abstract: The carbon backbone of biotin is constructed from the C7 di‐acid pimelate, which is converted to an acyl‐CoA thioester by an ATP‐dependent, pimeloyl‐CoA synthetase (PCAS, encoded by BioW). The acyl‐thioester is condensed with ʟ‐alanine in… read more here.
Keywords: fusion; biotin; domain; substrate ... See more keywords
Recombinant l‐Amino Acid Oxidase with Broad Substrate Spectrum for Co‐substrate Recycling in (S)‐Selective Transaminase‐Catalyzed Kinetic Resolutions
Sign Up to like & getrecommendations! Published in 2022 at "Chembiochem"
DOI: 10.1002/cbic.202200329
Abstract: Chiral and enantiopure amines can be produced by enantioselective transaminases via kinetic resolution of amine racemates. This transamination reaction requires stoichiometric amounts of co‐substrate. A dual‐enzyme recycling system overcomes this limitation: l‐amino acid oxidases (LAAO)… read more here.
Keywords: substrate spectrum; amino acid; transaminase; substrate ... See more keywords
Multipurpose cellulases of Promicromonospora sp. VP111, with broad substrate specificity and tolerance properties.
Sign Up to like & getrecommendations! Published in 2023 at "Journal of basic microbiology"
DOI: 10.1002/jobm.202200679
Abstract: Cellulolytic actinobacterium, Promicromonospora sp. VP111 concomitantly produced cellulases (CELs), xylanase and pectinase when grown on commercial cellulose and untreated agricultural lignocellulosic residues (wheat straw and sugarcane bagasse). Secreted CELs hydrolyzed (enhanced with Co2+ ion) multiple… read more here.
Keywords: substrate specificity; tolerance; promicromonospora vp111; broad substrate ... See more keywords
Biochemical characteristics of three feruloyl esterases with a broad substrate spectrum from Bacillus amyloliquefaciens H47
Sign Up to like & getrecommendations! Published in 2017 at "Process Biochemistry"
DOI: 10.1016/j.procbio.2016.12.012
Abstract: Abstract Feruloyl esterases (Faes) are a subclass of the carboxylic esterases that hydrolyze the ester bonds between ferulic acid and polysaccharides in plant cell walls. Until now, the biochemical characteristics of FAEs from Bacillus spp.… read more here.
Keywords: broad substrate; biochemical characteristics; feruloyl esterases; amyloliquefaciens h47 ... See more keywords
Structural basis for the broad substrate specificity of the human tyrosylprotein sulfotransferase-1
Sign Up to like & getrecommendations! Published in 2017 at "Scientific Reports"
DOI: 10.1038/s41598-017-07141-8
Abstract: Tyrosylprotein sulfotransferases (TPSTs) are enzymes that catalyze post-translational tyrosine sulfation of proteins. In humans, there are only two TPST isoforms, designated TPST1 and TPST2. In a previous study, we reported the crystal structure of TPST2,… read more here.
Keywords: structural basis; broad substrate; tyrosylprotein; sulfation ... See more keywords
A new carboxypeptidase from Aspergillus niger with good thermostability, pH stability and broad substrate specificity
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DOI: 10.1038/s41598-021-98003-x
Abstract: A new serine carboxypeptidase gene, capA, was identified in Aspergillus niger CBS 513.88 by reading genomic information and performing sequence alignment, and the gene was cloned and expressed in Pichia pastoris GS115. In a shake… read more here.
Keywords: aspergillus niger; mmol; capa; broad substrate ... See more keywords
Exploring Class I polyhydroxyalkanoate synthases with broad substrate specificity for polymerization of structurally diverse monomer units
Sign Up to like & getrecommendations! Published in 2023 at "Frontiers in Bioengineering and Biotechnology"
DOI: 10.3389/fbioe.2023.1114946
Abstract: Polyhydroxyalkanoate (PHA) synthases (PhaCs) are key enzymes in PHA polymerization. PhaCs with broad substrate specificity are attractive for synthesizing structurally diverse PHAs. In the PHA family, 3-hydroxybutyrate (3HB)-based copolymers are industrially produced using Class I… read more here.
Keywords: phacs; class; broad substrate; substrate specificity ... See more keywords
Isolation of a Highly Thermostable Bile Salt Hydrolase With Broad Substrate Specificity From Lactobacillus paragasseri
Sign Up to like & getrecommendations! Published in 2022 at "Frontiers in Microbiology"
DOI: 10.3389/fmicb.2022.810872
Abstract: Bile salt hydrolase (BSH) enzymes produced by intestinal Lactobacillus species have been recognized as major targets for probiotic studies owing to their weight-loss and cholesterol-lowering effects. In this study, we isolated a highly thermostable BSH… read more here.
Keywords: highly thermostable; broad substrate; bsh; substrate specificity ... See more keywords