Crystalline Sponge Method Enabled the Investigation of a Prenyltransferase-terpene Synthase Chimeric Enzyme, Whose Product Exhibits Broadened NMR Signals.
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DOI: 10.1021/acs.orglett.8b02284
Abstract: By the genome-mining approach, a chimeric enzyme of prenyltransferase-diterpene synthase was discovered from Penicillium chrysogenum MT-12. Since its product exhibited broadened NMR signals, the structural determination by only the NMR analysis was difficult, but the… read more here.
Keywords: sponge method; crystalline sponge; chimeric enzyme; broadened nmr ... See more keywords