Analysis of Tagged Proteins Using Tandem Affinity-Buffer Exchange Chromatography Online with Native Mass Spectrometry.
Sign Up to like & getrecommendations! Published in 2021 at "Biochemistry"
DOI: 10.1021/acs.biochem.1c00138
Abstract: Protein overexpression and purification are critical for in vitro structure-function characterization studies. However, some proteins are difficult to express in heterologous systems due to host-related (e.g., codon usage, translation rate) and/or protein-specific (e.g., toxicity, aggregation)… read more here.
Keywords: chromatography; native mass; buffer exchange; mass spectrometry ... See more keywords
Buffer Exchange, a Simple Option That Significantly Increases the Chances of Isolation of Adenoviruses from Blood and Other Clinical Specimens
Sign Up to like & getrecommendations! Published in 2023 at "Microbiology Spectrum"
DOI: 10.1128/spectrum.00454-23
Abstract: to process blood specimens (whole blood, plasma, occasionally serum) to attempt the isolation and characterization of human adenoviruses (HAdVs) infecting pediatric trans-plant recipients, we have repeatedly been challenged by the high toxicity of these samples… read more here.
Keywords: simple option; exchange simple; whole blood; isolation ... See more keywords