φC31 -Mediated Cassette Exchange in Sf9 Insect Cells for Stable Expression.
Sign Up to like & getrecommendations! Published in 2023 at "Biotechnology journal"
DOI: 10.1002/biot.202200557
Abstract: Insect cells, especially Sf9 cells, are commonly used in biomanufacturing due to their advantages in high expression levels and post-translational modification. However, the development of stable expression cell lines via random integration tended to be… read more here.
Keywords: cell; expression; c31; cassette exchange ... See more keywords
An att site-based recombination reporter system for genome engineering and synthetic DNA assembly
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DOI: 10.1186/s12896-017-0382-1
Abstract: BackgroundDirect manipulation of the genome is a widespread technique for genetic studies and synthetic biology applications. The tyrosine and serine site-specific recombination systems of bacteriophages HK022 and ΦC31 are widely used for stable directional exchange… read more here.
Keywords: system; dna; bla; c31 ... See more keywords
Synthesis and X-ray Crystal Structure of New Substituted 3-4′-Bipyrazole Derivatives. Hirshfeld Analysis, DFT and NBO Studies
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DOI: 10.3390/cryst11080953
Abstract: A new compounds named 3-4′-bipyrazoles 2 and 3 were synthesized in high chemical yield from a reaction of pyran-2,4-diketone 1 with aryl hydrazines under thermal conditions in MeOH. Compound 2 was unambiguously confirmed by single-crystal… read more here.
Keywords: crystal structure; kcal mol; analysis; c31 ... See more keywords
Comparative Transcriptome Profiling Reveals Potential Candidate Genes, Transcription Factors, and Biosynthetic Pathways for Phosphite Response in Potato (Solanum tuberosum L.)
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DOI: 10.3390/genes13081379
Abstract: The study was conducted with C31 and C80 genotypes of the potato (Solanum tuberosum L.), which are tolerant and susceptible to phosphite (Phi, H2PO3), respectively. To decipher the molecular mechanisms underlying tolerance and susceptibility to… read more here.
Keywords: potato solanum; c80 phi; phi; c31 ... See more keywords