Dynamic Ca2+ imaging with a simplified lattice light-sheet microscope: A sideways view of subcellular Ca2+ puffs.
Sign Up to like & getrecommendations! Published in 2018 at "Cell calcium"
DOI: 10.1016/j.ceca.2017.11.005
Abstract: We describe the construction of a simplified, inexpensive lattice light-sheet microscope, and illustrate its use for imaging subcellular Ca2+ puffs evoked by photoreleased i-IP3 in cultured SH-SY5Y neuroblastoma cells loaded with the Ca2+ probe Cal520.… read more here.
Keywords: ca2 puffs; microscope; lattice light; ca2 ... See more keywords
KRAP tethers IP3 receptors to actin and licenses them to evoke cytosolic Ca2+ signals
Sign Up to like & getrecommendations! Published in 2021 at "Nature Communications"
DOI: 10.1038/s41467-021-24739-9
Abstract: Regulation of IP3 receptors (IP3Rs) by IP3 and Ca2+ allows regenerative Ca2+ signals, the smallest being Ca2+ puffs, which arise from coordinated openings of a few clustered IP3Rs. Cells express thousands of mostly mobile IP3Rs,… read more here.
Keywords: ca2 signals; krap tethers; ca2 puffs; ca2 ... See more keywords
Endogenous signalling pathways and caged IP3 evoke Ca2+ puffs at the same abundant immobile intracellular sites
Sign Up to like & getrecommendations! Published in 2017 at "Journal of Cell Science"
DOI: 10.1242/jcs.208520
Abstract: ABSTRACT The building blocks of intracellular Ca2+ signals evoked by inositol 1,4,5-trisphosphate receptors (IP3Rs) are Ca2+ puffs, transient focal increases in Ca2+ concentration that reflect the opening of small clusters of IP3Rs. We use total… read more here.
Keywords: ca2 puffs; signalling pathways; caged ip3; evoked ca2 ... See more keywords