Potential Activation Mechanism of Calcium Indicator WHaloCaMP1a Revealed by the Crystal Structure and Molecular Dynamics Simulations.
Sign Up to like & getrecommendations! Published in 2025 at "Biochemistry"
DOI: 10.1021/acs.biochem.5c00352
Abstract: Chemigenetic indicators combined with advanced organic fluorophores have become increasingly popular in biosensor development due to their integration of protein biocompatibility and the superior optical properties of dyes. However, despite the development of various biosensors… read more here.
Keywords: crystal structure; indicator whalocamp1a; dynamics simulations; activation ... See more keywords
High-throughput-compatible assays using a genetically-encoded calcium indicator
Sign Up to like & getrecommendations! Published in 2019 at "Scientific Reports"
DOI: 10.1038/s41598-019-49070-8
Abstract: Measurement of intracellular calcium in live cells is a key component of a wide range of basic life science research, and crucial for many high-throughput assays used in modern drug discovery. Synthetic calcium indicators have… read more here.
Keywords: calcium indicator; synthetic calcium; calcium indicators; calcium ... See more keywords
A sensitive soma-localized red fluorescent calcium indicator for in vivo imaging of neuronal populations at single-cell resolution
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DOI: 10.1371/journal.pbio.3003048
Abstract: Recent advancements in genetically encoded calcium indicators, particularly those based on green fluorescent proteins, have optimized their performance for monitoring neuronal activities in a variety of model organisms. However, progress in developing red-shifted GECIs, despite… read more here.
Keywords: soma; neuronal populations; sensitive soma; calcium indicator ... See more keywords
Bicistronic Expression of a High-Performance Calcium Indicator and Opsin for All-Optical Stimulation and Imaging at Cellular Resolution
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DOI: 10.1523/eneuro.0378-22.2023
Abstract: Abstract State-of-the-art all-optical systems promise unprecedented access to neural activity in vivo, using multiphoton optogenetics to allow simultaneous imaging and control of activity in selected neurons at cellular resolution. However, to achieve wide use of… read more here.
Keywords: cellular resolution; optical stimulation; stimulation; stimulation imaging ... See more keywords