Deleterious effects of calcium indicators within cells; an inconvenient truth.
Sign Up to like & getrecommendations! Published in 2018 at "Cell calcium"
DOI: 10.1016/j.ceca.2018.04.005
Abstract: The study of cellular Ca2+ signalling is indebted to Roger Tsien for the invention of fluorescent indicators that can be readily loaded into living cells and provide the means to measure cellular Ca2+ changes over… read more here.
Keywords: within cells; effects calcium; indicators within; cells inconvenient ... See more keywords
The GCaMP-R Family of Genetically Encoded Ratiometric Calcium Indicators.
Sign Up to like & getrecommendations! Published in 2017 at "ACS chemical biology"
DOI: 10.1021/acschembio.6b00883
Abstract: We report on GCaMP-Rs, a new family of genetically encoded ratiometric calcium indicators that extend the virtues of the GCaMP proteins to ratiometric measurements. We have engineered a tandem construct of calcium-dependent GCaMP and calcium-independent… read more here.
Keywords: encoded ratiometric; genetically encoded; ratiometric calcium; calcium indicators ... See more keywords
High-throughput-compatible assays using a genetically-encoded calcium indicator
Sign Up to like & getrecommendations! Published in 2019 at "Scientific Reports"
DOI: 10.1038/s41598-019-49070-8
Abstract: Measurement of intracellular calcium in live cells is a key component of a wide range of basic life science research, and crucial for many high-throughput assays used in modern drug discovery. Synthetic calcium indicators have… read more here.
Keywords: calcium indicator; synthetic calcium; calcium indicators; calcium ... See more keywords
Genetically Encoded Calcium Indicators for Functional Imaging of Mosquito Olfactory Neurons.
Sign Up to like & getrecommendations! Published in 2022 at "Cold Spring Harbor protocols"
DOI: 10.1101/pdb.top107683
Abstract: Mosquitoes transmit a multitude of diseases to humans and animals through biting and blood feeding. To locate their hosts, mosquitoes primarily use their sense of smell. Therefore, an understanding of mosquito olfaction will help develop… read more here.
Keywords: calcium; olfactory neurons; encoded calcium; mosquito olfactory ... See more keywords
Transcriptional Genetically Encoded Calcium Indicators in Drosophila.
Sign Up to like & getrecommendations! Published in 2022 at "Cold Spring Harbor protocols"
DOI: 10.1101/pdb.top107797
Abstract: Knowing which neurons are active during behavior is a crucial step toward understanding how nervous systems work. Neuronal activation is generally accompanied by an increase in intracellular calcium levels. Therefore, intracellular calcium levels are widely… read more here.
Keywords: intracellular calcium; calcium; calcium levels; encoded calcium ... See more keywords
cNTnC and fYTnC2, Genetically Encoded Green Calcium Indicators Based on Troponin C from Fast Animals
Sign Up to like & getrecommendations! Published in 2022 at "International Journal of Molecular Sciences"
DOI: 10.3390/ijms232314614
Abstract: NTnC-like green fluorescent genetically encoded calcium indicators (GECIs) with two calcium ion binding sites were constructed using the insertion of truncated troponin C (TnC) from Opsanus tau into green fluorescent proteins (GFPs). These GECIs are… read more here.
Keywords: calcium; cntnc fytnc2; fast animals; calcium indicators ... See more keywords