Site‐specifically Incorporated Non‐Canonical Amino Acids into Pseudomonas alcaligenes Lipase to Hydrolyze L‐menthol Propionate among the Eight Isomers
Sign Up to like & getrecommendations! Published in 2021 at "ChemCatChem"
DOI: 10.1002/cctc.202100358
Abstract: It remains a challenge to improve the diastereopreference of enzymes when there are multiple chiral centers in the substrate, mainly because the limited understanding of mechanism determining diastereoselectivity. Compared with natural amino acids, non‐canonical amino… read more here.
Keywords: non canonical; canonical amino; amino acids; menthol propionate ... See more keywords
From Folding to Assembly: Functional Supramolecular Architectures of Peptides Comprised of Non-Canonical Amino Acids.
Sign Up to like & getrecommendations! Published in 2021 at "Macromolecular bioscience"
DOI: 10.1002/mabi.202100090
Abstract: The engineering of biological molecules is the fundamental concept behind the design of complex materials with desirable functions. Over the last few decades, peptides and proteins have emerged as useful building blocks for well-defined nanostructures… read more here.
Keywords: amino acids; folding assembly; non canonical; canonical amino ... See more keywords
Generating Efficient Methanomethylophilus alvus Pyrrolysyl-tRNA Synthetases for Structurally Diverse Non-Canonical Amino Acids.
Sign Up to like & getrecommendations! Published in 2022 at "ACS chemical biology"
DOI: 10.1021/acschembio.2c00639
Abstract: Genetic code expansion (GCE) technologies commonly use the pyrrolysyl-tRNA synthetase (PylRS)/tRNAPyl pairs from Methanosarcina mazei (Mm) and Methanosarcina barkeri (Mb) for site-specific incorporation of non-canonical amino acids (ncAAs) into proteins. Recently a homologous PylRS/tRNAPyl pair… read more here.
Keywords: pylrs; non canonical; canonical amino; pyrrolysyl trna ... See more keywords
Optimized Bioorthogonal Non-canonical Amino Acid Tagging to Identify Serotype-Specific Biomarkers in Verotoxigenic Escherichia coli.
Sign Up to like & getrecommendations! Published in 2023 at "ACS infectious diseases"
DOI: 10.1021/acsinfecdis.2c00548
Abstract: According to Canada's Food Report Card 2016, there are 4 million foodborne illnesses acquired each year in the nation alone. The leading causes of foodborne illness are pathogenic bacteria such as shigatoxigenic/verotoxigenic Escherichia coli (STEC/VTEC)… read more here.
Keywords: escherichia coli; non canonical; canonical amino; bioorthogonal non ... See more keywords
Beyond 20 in the 21st Century: Prospects and Challenges of Non-canonical Amino Acids in Peptide Drug Discovery.
Sign Up to like & getrecommendations! Published in 2023 at "ACS medicinal chemistry letters"
DOI: 10.1021/acsmedchemlett.3c00037
Abstract: Life is constructed primarily using a toolbox of 20 canonical amino acids-relying upon these building blocks for the assembly of proteins and peptides that regulate nearly every cellular task, including cell structure, function, and maintenance.… read more here.
Keywords: amino acids; drug; canonical amino; drug discovery ... See more keywords
A 68-codon genetic code to incorporate four distinct non-canonical amino acids enabled by automated orthogonal mRNA design
Sign Up to like & getrecommendations! Published in 2021 at "Nature Chemistry"
DOI: 10.1038/s41557-021-00764-5
Abstract: Orthogonal (O) ribosome-mediated translation of O-mRNAs enables the incorporation of up to three distinct non-canonical amino acids (ncAAs) into proteins in Escherichia coli (E. coli). However, the general and efficient incorporation of multiple distinct ncAAs… read more here.
Keywords: four distinct; amino acids; non canonical; canonical amino ... See more keywords
Chemically-defined lactose-based autoinduction medium for site-specific incorporation of non-canonical amino acids into proteins
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DOI: 10.1039/c8ra04359k
Abstract: Optimized chemically-defined lactose-based autoinduction media for superior expression levels of proteins with non-canonical amino acids. read more here.
Keywords: based autoinduction; non canonical; defined lactose; lactose based ... See more keywords
Employing non-canonical amino acids towards the immobilization of a hyperthermophilic enzyme to increase protein stability
Sign Up to like & getrecommendations! Published in 2023 at "RSC Advances"
DOI: 10.1039/d3ra00392b
Abstract: A carboxylesterase derived from Sulfolobus solfataricus P1 was immobilized onto an epoxy-activated Sepharose resin via non-canonical amino acids. The immobilized enzyme exhibited heightened performance in organic solvents, recyclability, and stability at room temperature for over… read more here.
Keywords: immobilization; amino acids; non canonical; canonical amino ... See more keywords
Mechanisms and minimization of false discovery of metabolic bio-orthogonal non-canonical amino acid proteomics.
Sign Up to like & getrecommendations! Published in 2022 at "Rejuvenation research"
DOI: 10.1089/rej.2022.0019
Abstract: Metabolic proteomics has been widely used to characterize dynamic protein networks in many areas of biomedicine, including in the arena of tissue aging and rejuvenation. Bio-orthogonal non-canonical amino acid tagging (BONCAT) is based on mutant… read more here.
Keywords: bio orthogonal; orthogonal non; false discovery; non canonical ... See more keywords
Identification of permissive amber suppression sites for efficient non-canonical amino acid incorporation in mammalian cells
Sign Up to like & getrecommendations! Published in 2021 at "Nucleic Acids Research"
DOI: 10.1093/nar/gkab132
Abstract: Abstract The genetic code of mammalian cells can be expanded to allow the incorporation of non-canonical amino acids (ncAAs) by suppressing in-frame amber stop codons (UAG) with an orthogonal pyrrolysyl-tRNA synthetase (PylRS)/tRNAPylCUA (PylT) pair. However,… read more here.
Keywords: incorporation; ncaa incorporation; non canonical; canonical amino ... See more keywords
Evidence for co-translational misincorporation of non-canonical amino acid hydroxyproline in recombinant antibodies produced in Chinese Hamster Ovary (CHO) cell lines
Sign Up to like & getrecommendations! Published in 2020 at "PLoS ONE"
DOI: 10.1371/journal.pone.0241250
Abstract: With the advent of highly sensitive technologies such as tandem mass spectrometry and next-generation sequencing, recombinant antibodies are now routinely analyzed for the presence of low-level sequence variants including amino acid misincorporations. During mAb cell… read more here.
Keywords: translational misincorporation; amino acid; hydroxyproline; non canonical ... See more keywords