Proper cytoskeleton α‐tubulin distribution is concomitant to tyrosine phosphorylation during in vitro capacitation and acrosomal reaction in human spermatozoa
Sign Up to like & getrecommendations! Published in 2020 at "Cytoskeleton"
DOI: 10.1002/cm.21631
Abstract: Spermatozoa motility is a key parameter during the fertilization process. In this context, spermatozoa tyrosine protein phosphorylation and an appropriate cytoskeleton α‐tubulin distribution are some of the most important physiological events involved in motility. However,… read more here.
Keywords: spermatozoa; capacitation; tyrosine; tubulin ... See more keywords
Detection of extracellular vesicles in the mouse vaginal fluid: Their delivery of sperm proteins that stimulate capacitation and modulate fertility
Sign Up to like & getrecommendations! Published in 2019 at "Journal of Cellular Physiology"
DOI: 10.1002/jcp.27894
Abstract: Extracellular vesicles (EVs) were isolated by ultracentrifugation of vaginal luminal fluid (VLF) from superovulated mice and identified for the first time using transmission electron microscopy. Characterized by size and biochemical markers (CD9 and HSC70), EVs… read more here.
Keywords: extracellular vesicles; fluid; capacitation; detection extracellular ... See more keywords
Testis‐specific isoform of angiotensin‐converting enzyme (tACE) is involved in the regulation of bovine sperm capacitation
Sign Up to like & getrecommendations! Published in 2017 at "Molecular Reproduction and Development"
DOI: 10.1002/mrd.22790
Abstract: We hypothesized that the testis‐specific isoform of angiotensin‐converting enzyme (tACE) is released during bovine sperm capacitation, and its peptidase activity is required for capacitation. Specific objectives of this study were to (i) develop an anti‐tACE… read more here.
Keywords: bovine sperm; sperm capacitation; tace; specific isoform ... See more keywords
Prediction of male fertility using capacitation‐associated proteins in spermatozoa
Sign Up to like & getrecommendations! Published in 2017 at "Molecular Reproduction and Development"
DOI: 10.1002/mrd.22810
Abstract: Infertility and subfertility account for huge economic losses in the animal industry; indeed, 50% of animal breeding failure is associated with male infertility. Approximately 70% of cattle and 90% of pig livestock are currently produced… read more here.
Keywords: capacitation; prediction male; fertility using; male fertility ... See more keywords
Iberiotoxin and clofilium regulate hyperactivation, acrosome reaction, and ion homeostasis synergistically during human sperm capacitation
Sign Up to like & getrecommendations! Published in 2023 at "Molecular Reproduction and Development"
DOI: 10.1002/mrd.23671
Abstract: Potassium channels play essential roles in the regulation of male fertility. However, potassium channels mediating K+ currents in human sperm (IKSper) remain controversial. Besides SLO3, the SLO1 potassium channel is a potential candidate for human… read more here.
Keywords: capacitation; hyperactivation; clofilium; acrosome reaction ... See more keywords
SERPINA5 Protein in Cumulus-Oocyte Complexes Increases the Fertilisation Ability of Mouse Sperm
Sign Up to like & getrecommendations! Published in 2022 at "Reproductive Sciences"
DOI: 10.1007/s43032-022-00867-5
Abstract: Obtaining high-quality sperm is key to improving the success rate of assisted reproductive technology (ART). Although cytokines secreted by cumulus-oocyte complexes (COCs) bind to sperm surface receptors to improve sperm quality, the effects of adding… read more here.
Keywords: capacitation; fertilisation; sperm; serpina5 protein ... See more keywords
A membrane-associated adenylate cyclase modulates lactate dehydrogenase and creatine kinase activities required for bull sperm capacitation induced by hyaluronic acid.
Sign Up to like & getrecommendations! Published in 2017 at "Animal reproduction science"
DOI: 10.1016/j.anireprosci.2017.02.004
Abstract: Hyaluronic acid, as well as heparin, is a glycosaminoglycan present in the female genital tract of cattle. The aim of this study was to evaluate oxidative metabolism and intracellular signals mediated by a membrane-associated adenylate… read more here.
Keywords: sperm capacitation; capacitation; bull sperm; hyaluronic acid ... See more keywords
Evaluation of boar and bull sperm capacitation and the acrosome reaction using flow cytometry.
Sign Up to like & getrecommendations! Published in 2021 at "Animal reproduction science"
DOI: 10.1016/j.anireprosci.2021.106846
Abstract: Flow cytometry can be used to evaluate many sperm attributes and Dr. Duane Garner was influential in developing assays to understand sperm physiology and function. We review some of Dr. Garner's work and describe experiments… read more here.
Keywords: sperm capacitation; capacitation; philosophy; physiology ... See more keywords
Luteinizing hormone modulates intracellular calcium, protein tyrosine phosphorylation and motility during human sperm capacitation.
Sign Up to like & getrecommendations! Published in 2017 at "Biochemical and biophysical research communications"
DOI: 10.1016/j.bbrc.2017.01.010
Abstract: In order to fertilize, spermatozoa must undergo physiological and biochemical changes during their transit along the female reproductive tract before reaching and fusing with the oocyte, process known as capacitation. Sperm modifications associated with capacitation… read more here.
Keywords: hormone; capacitation; protein tyrosine; motility ... See more keywords
Carnitine supplementation decreases capacitation-like changes of frozen-thawed buffalo spermatozoa.
Sign Up to like & getrecommendations! Published in 2017 at "Theriogenology"
DOI: 10.1016/j.theriogenology.2016.09.031
Abstract: The aim of this study was to evaluate the effect of carnitine supplementation of semen extender on fertility parameters of frozen-thawed buffalo sperm. Buffalo semen was cryopreserved in BioXcell containing 0 (control group), 2.5 and… read more here.
Keywords: frozen thawed; thawed buffalo; capacitation; carnitine ... See more keywords
Sperm-bound antisperm antibodies prevent capacitation of bovine spermatozoa.
Sign Up to like & getrecommendations! Published in 2017 at "Theriogenology"
DOI: 10.1016/j.theriogenology.2016.10.012
Abstract: It was hypothesized here that sperm-bound antisperm antibodies (ASAs) impair the ability of bovine spermatozoa to undergo capacitation, bind to the zona pellucida, and complete the acrosome reaction. The effect of ASA binding on these… read more here.
Keywords: bovine spermatozoa; iga; spermatozoa; capacitation ... See more keywords