Design of experiments as a valuable tool for biopharmaceutical analysis with (imaged) capillary isoelectric focusing
Sign Up to like & getrecommendations! Published in 2019 at "ELECTROPHORESIS"
DOI: 10.1002/elps.201900162
Abstract: Capillary isoelectric focusing is indispensable for characterizing charge heterogeneity and isoelectric points of biopharmaceuticals. However, there are many influencing parameters and therefore method development is challenging. This study was performed to obtain an in‐depth understanding… read more here.
Keywords: capillary isoelectric; experiments valuable; valuable tool; design experiments ... See more keywords
Analysis of four therapeutic monoclonal antibodies by online capillary isoelectric focusing directly coupled to quadrupole time‐of‐flight mass spectrometry
Sign Up to like & getrecommendations! Published in 2019 at "ELECTROPHORESIS"
DOI: 10.1002/elps.201900195
Abstract: Capillary isoelectric focusing (cIEF) was online coupled to a Q‐TOF MS by a flow‐through microvial interface for the analysis of therapeutic mAb. Intact molecular weights obtained from the mass spectrum deconvolution of separated charge variants… read more here.
Keywords: capillary isoelectric; analysis four; analysis; mass ... See more keywords
Electrophoretic mobilization in capillary isoelectric focusing by a weak acid or an acidic ampholyte as catholyte assessed by computer simulation
Sign Up to like & getrecommendations! Published in 2022 at "ELECTROPHORESIS"
DOI: 10.1002/elps.202200262
Abstract: Capillary isoelectric focusing (CIEF) with cationic electrophoretic mobilization induced via replacing the catholyte with the anolyte or a solution of another acid or amino acid was investigated by computer simulation for a wide range pH… read more here.
Keywords: isoelectric focusing; computer simulation; electrophoretic mobilization; acid ... See more keywords
Comparison between capillary zone electrophoresis and capillary isoelectric focusing for thalassemia screening in southern China
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DOI: 10.1002/jcla.22567
Abstract: Capillary isoelectric focusing is a type of capillary electrophoresis method newly used for thalassemia screening in China. Although the good performance has been proved by several studies, whether it can best suit the special needs… read more here.
Keywords: capillary isoelectric; comparison capillary; electrophoresis; thalassemia screening ... See more keywords
Automated Capillary Isoelectric Focusing-Mass Spectrometry with Ultrahigh Resolution for Characterizing Microheterogeneity and Isoelectric Points of Intact Protein Complexes.
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DOI: 10.1021/acs.analchem.2c00975
Abstract: Protein complexes are the functional machines in the cell and are heterogeneous due to protein sequence variations and post-translational modifications (PTMs). Here, we present an automated nondenaturing capillary isoelectric focusing-mass spectrometry (ncIEF-MS) methodology for uncovering… read more here.
Keywords: protein complexes; isoelectric focusing; mass spectrometry; focusing mass ... See more keywords
Highly sensitive and specific protein detection via combined capillary isoelectric focusing and proximity ligation
Sign Up to like & getrecommendations! Published in 2017 at "Scientific Reports"
DOI: 10.1038/s41598-017-01516-7
Abstract: Detection and quantification of proteins and their post-translational modifications are crucial to decipher functions of complex protein networks in cell biology and medicine. Capillary isoelectric focusing together with antibody-based detection can resolve and identify proteins… read more here.
Keywords: protein; detection; proximity ligation; capillary isoelectric ... See more keywords