Sourcing Phage-Encoded Terminators Using ONT-cappable-seq for SynBio Applications in Pseudomonas
Sign Up to like & getrecommendations! Published in 2023 at "ACS Synthetic Biology"
DOI: 10.1021/acssynbio.3c00101
Abstract: Efficient transcriptional terminators are essential for the performance of genetic circuitry in microbial SynBio hosts. In recent years, several libraries of characterized strong terminators have become available for model organisms such as Escherichia coli. Conversely,… read more here.
Keywords: ont cappable; phage encoded; sourcing phage; synbio ... See more keywords
SMRT-Cappable-seq reveals complex operon variants in bacteria
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DOI: 10.1038/s41467-018-05997-6
Abstract: Current methods for genome-wide analysis of gene expression require fragmentation of original transcripts into small fragments for short-read sequencing. In bacteria, the resulting fragmented information hides operon complexity. Additionally, in vivo processing of transcripts confounds the… read more here.
Keywords: operon; smrt cappable; cappable seq; operon variants ... See more keywords
The primary transcriptome of hormogonia from a filamentous cyanobacterium defined by cappable-seq.
Sign Up to like & getrecommendations! Published in 2021 at "Microbiology"
DOI: 10.1099/mic.0.001111
Abstract: Hormogonia are motile filaments produced by many filamentous cyanobacteria that function in dispersal, phototaxis and the establishment of nitrogen-fixing symbioses. The gene regulatory network promoting hormogonium development is initiated by the hybrid histidine kinase HrmK,… read more here.
Keywords: primary transcriptome; sigj; filamentous cyanobacterium; cappable seq ... See more keywords
Genome-scale analyses of transcriptional start sites in Mycobacterium marinum under normoxic and hypoxic conditions
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DOI: 10.1186/s12864-021-07572-8
Abstract: Background Hypoxic stress plays a critical role in the persistence of Mycobacterium tuberculosis (Mtb) infection, but the mechanisms underlying this adaptive response remain ill defined. Material and methods In this study, using M. marinum as… read more here.
Keywords: seq; hypoxic conditions; transcriptional start; cappable seq ... See more keywords
Cappable-Seq Reveals Specific Patterns of Metabolism and Virulence for Salmonella Typhimurium Intracellular Survival within Acanthamoeba castellanii
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DOI: 10.3390/ijms22169077
Abstract: The bacterial pathogen Salmonella enterica, which causes enteritis, has a broad host range and extensive environmental longevity. In water and soil, Salmonella interacts with protozoa and multiplies inside their phagosomes. Although this relationship resembles that… read more here.
Keywords: salmonella; castellanii; acanthamoeba castellanii; cappable seq ... See more keywords