Enhancing the production of adeno‐associated virus (AAV)2 and AAV9 with high full capsid ratio in HEK293 cells through design‐of‐experiment optimization of triple plasmid ratio
Sign Up to like & getrecommendations! Published in 2024 at "Biotechnology Journal"
DOI: 10.1002/biot.202300667
Abstract: The recombinant adeno‐associated virus (rAAV) vectors used in gene therapy are usually produced by transfecting three different plasmids (Adenoviral helper plasmid (pHelper), AAV rep/cap plasmids (pRepCap), and Transgene plasmid (pAAV‐GOI)) into human embryonic kidney 293… read more here.
Keywords: capsid; hek293 cells; plasmid ratio; plasmid ... See more keywords
Multidose transient transfection of human embryonic kidney 293 cells modulates recombinant adeno‐associated virus2/5 Rep protein expression and influences the enrichment fraction of filled capsids
Sign Up to like & getrecommendations! Published in 2024 at "Biotechnology and Bioengineering"
DOI: 10.1002/bit.28828
Abstract: Recombinant adeno‐associated virus (rAAV) is a commonly used in vivo gene therapy vector because of its nonpathogenicity, long‐term transgene expression, broad tropism, and ability to transduce both dividing and nondividing cells. However, rAAV vector production… read more here.
Keywords: recombinant adeno; capsid; transfection; expression ... See more keywords
High-Mass MALDI-MS Analysis for the Investigation of Protein Encapsulation within an Engineered Capsid Forming Protein
Sign Up to like & getrecommendations! Published in 2017 at "Helvetica Chimica Acta"
DOI: 10.1002/hlca.201700166
Abstract: Chemical cross-linking combined with MALDI-MS was applied to structural analysis of a protein nanocontainer. Specifically, an engineered variant of lumazine synthase from Aquifex aeolicus (AaLS-13) was investigated that self-assembles into a capsid-like structure and is… read more here.
Keywords: protein; analysis; mass maldi; capsid ... See more keywords
SERTAD3 induces proteasomal degradation of ZIKV capsid protein and represents a therapeutic target
Sign Up to like & getrecommendations! Published in 2023 at "Journal of Medical Virology"
DOI: 10.1002/jmv.28451
Abstract: Zika virus (ZIKV) is a mosquito‐borne RNA virus that belongs to the Flaviviridae family. While flavivirus replication is known to occur in the cytoplasm, a significant portion of the viral capsid protein localizes to the… read more here.
Keywords: capsid protein; zikv; capsid; proteasomal degradation ... See more keywords
Changes in dynamic and static structures of the HIV‐1 p24 capsid protein N‐domain caused by amino‐acid substitution are associated with its viral viability
Sign Up to like & getrecommendations! Published in 2021 at "Protein Science"
DOI: 10.1002/pro.4184
Abstract: HIV‐1 capsid is comprised of over a hundred p24 protein molecules, arranged as either pentamers or hexamers. Three p24 mutants with amino acid substitutions in capsid N‐terminal domain protein were examined: G60W (α3‐4 loop), M68T… read more here.
Keywords: protein; amino acid; conformation; capsid ... See more keywords
Global and regional circulation trends of norovirus genotypes and recombinants, 1995–2019: A comprehensive review of sequences from public databases
Sign Up to like & getrecommendations! Published in 2022 at "Reviews in Medical Virology"
DOI: 10.1002/rmv.2354
Abstract: Human noroviruses are the leading global cause of viral gastroenteritis. Attempts at developing effective vaccines and treatments against norovirus disease have been stymied by the extreme genetic diversity and rapid geographic distribution of these viruses.… read more here.
Keywords: 1995 2019; gii p16; public databases; capsid ... See more keywords
Combination of hydrophilic interaction liquid chromatography and top-down mass spectrometry for characterisation of adeno-associated virus capsid proteins
Sign Up to like & getrecommendations! Published in 2025 at "Analytical and Bioanalytical Chemistry"
DOI: 10.1007/s00216-025-05874-4
Abstract: Adeno-associated virus (AAV) viral vector-based gene therapy is advancing rapidly, offering potential treatments for rare and severe diseases. The AAV capsid consists of a combination of three viral proteins (VPs), VP1, VP2, and VP3, ranging… read more here.
Keywords: adeno associated; capsid; characterisation; capsid proteins ... See more keywords
Generation in yeast and antigenic characterization of hepatitis E virus capsid protein virus-like particles
Sign Up to like & getrecommendations! Published in 2017 at "Applied Microbiology and Biotechnology"
DOI: 10.1007/s00253-017-8622-9
Abstract: Hepatitis E is a globally distributed human disease caused by hepatitis E virus (HEV). In Europe, it spreads through undercooked pork meat or other products and with blood components through transfusions. There are no approved… read more here.
Keywords: rat hev; hev capsid; hev; capsid ... See more keywords
A broadly cross-reactive monoclonal antibody against hepatitis E virus capsid antigen
Sign Up to like & getrecommendations! Published in 2021 at "Applied Microbiology and Biotechnology"
DOI: 10.1007/s00253-021-11342-7
Abstract: Abstract To generate a hepatitis E virus (HEV) genotype 3 (HEV-3)–specific monoclonal antibody (mAb), the Escherichia coli–expressed carboxy-terminal part of its capsid protein was used to immunise BALB/c mice. The immunisation resulted in the induction… read more here.
Keywords: antibody; capsid protein; hev; capsid ... See more keywords
Structure-based virtual screening workflow to identify antivirals targeting HIV-1 capsid
Sign Up to like & getrecommendations! Published in 2022 at "Journal of Computer-Aided Molecular Design"
DOI: 10.1007/s10822-022-00446-5
Abstract: We have identified novel HIV-1 capsid inhibitors targeting the PF74 binding site. Acting as the building block of the HIV-1 capsid core, the HIV-1 capsid protein plays an important role in the viral life cycle… read more here.
Keywords: structure based; screening workflow; capsid; hiv capsid ... See more keywords
Molecular dynamics study of T = 3 capsid assembly
Sign Up to like & getrecommendations! Published in 2018 at "Journal of Biological Physics"
DOI: 10.1007/s10867-018-9486-7
Abstract: Molecular dynamics simulation is used to model the self-assembly of polyhedral shells containing 180 trapezoidal particles that correspond to the T = 3 virus capsid. Three kinds of particle, differing only slightly in shape, are… read more here.
Keywords: study capsid; dynamics study; molecular dynamics; capsid assembly ... See more keywords