Highly secretory expression of recombinant cowpea chlorotic mottle virus capsid proteins in Pichia pastoris and in-vitro encapsulation of ruthenium nanoparticles for catalysis.
Sign Up to like & getrecommendations! Published in 2020 at "Protein expression and purification"
DOI: 10.1016/j.pep.2020.105679
Abstract: The applications of viral protein cages have expanded rapidly into the fields of bionanotechnology and materials science. However, the low-cost production of viral capsid proteins (CPs) on a large scale is always a challenge. Herein,… read more here.
Keywords: chlorotic mottle; capsid proteins; cowpea chlorotic; expression ... See more keywords
Templated co-assembly into nanorods of polyanions and artificial virus capsid proteins.
Sign Up to like & getrecommendations! Published in 2017 at "Soft matter"
DOI: 10.1039/c7sm02012k
Abstract: Recombinant triblock polypeptides C-Sn-B, where C is a 400 amino acid long hydrophilic random coil block, Sn is a multimer of the silk-like octapeptide S = (GAGAGAGQ), and B = K12 is an oligolysine, have… read more here.
Keywords: templated assembly; capsid proteins; artificial virus; microscopy ... See more keywords
Cellular homologs of the double jelly-roll major capsid proteins clarify the origins of an ancient virus kingdom
Sign Up to like & getrecommendations! Published in 2022 at "Proceedings of the National Academy of Sciences of the United States of America"
DOI: 10.1073/pnas.2120620119
Abstract: Significance Viruses are the most abundant biological entities on Earth and ubiquitous parasites of cellular life forms. The general scenario for the origin of viruses involves evolution from nonviral replicators, such as plasmids and transposons,… read more here.
Keywords: capsid proteins; double jelly; capsid; roll major ... See more keywords
Direct Liquid Chromatography/Mass Spectrometry Analysis for Complete Characterization of Recombinant Adeno-Associated Virus Capsid Proteins.
Sign Up to like & getrecommendations! Published in 2017 at "Human gene therapy methods"
DOI: 10.1089/hgtb.2016.178
Abstract: The requirement for robust analytical methods to characterize adeno-associated virus (AAV) vectors is immediate, as the field advances more AAV gene therapies into the clinic and onto commercialization. AAV capsid proteins (VPs) are critical for… read more here.
Keywords: capsid proteins; mass spectrometry; analysis; liquid chromatography ... See more keywords
In Situ Structures of the Polymerase Complex and RNA Genome Show How Aquareovirus Transcription Machineries Respond to Uncoating
Sign Up to like & getrecommendations! Published in 2018 at "Journal of Virology"
DOI: 10.1128/jvi.00774-18
Abstract: Viruses in the family Reoviridae are characterized by the ability to endogenously synthesize nascent RNA within the virus. However, the mechanisms for assembling their RNA genomes with transcriptional enzymes into a multilayered virion and for… read more here.
Keywords: transcription; capsid proteins; rna; polymerase ... See more keywords
Identification of Epitopes on Rhinovirus 89 Capsid Proteins Capable of Inducing Neutralizing Antibodies
Sign Up to like & getrecommendations! Published in 2022 at "International Journal of Molecular Sciences"
DOI: 10.3390/ijms23095113
Abstract: Rhinoviruses (RVs) are major causes of the common cold, but they can also trigger exacerbations of asthma. More than 160 different RV strains exist and can be classified into three genetic species (RV-A, RV-B and… read more here.
Keywords: rv89; neutralizing antibodies; capsid proteins; group ... See more keywords
Boosting the Separation of Adeno-Associated Virus Capsid Proteins by Liquid Chromatography and Capillary Electrophoresis Approaches
Sign Up to like & getrecommendations! Published in 2023 at "International Journal of Molecular Sciences"
DOI: 10.3390/ijms24108503
Abstract: The purity of the three capsid proteins that make up recombinant adeno-associated virus (rAAV) is considered a critical quality attribute of gene therapy products. As such, there is a clear need to develop separation methods… read more here.
Keywords: capsid proteins; associated virus; adeno associated; capsid ... See more keywords
Exogenous Rubella Virus Capsid Proteins Enhance Virus Genome Replication
Sign Up to like & getrecommendations! Published in 2022 at "Pathogens"
DOI: 10.3390/pathogens11060683
Abstract: Enhanced replication of rubella virus (RuV) and replicons by de novo synthesized viral structural proteins has been previously described. Such enhancement can occur by viral capsid proteins (CP) alone in trans. It is not clear… read more here.
Keywords: capsid proteins; genome replication; replication; rna ... See more keywords
NMR Experiments Shed New Light on Glycan Recognition by Human and Murine Norovirus Capsid Proteins
Sign Up to like & getrecommendations! Published in 2021 at "Viruses"
DOI: 10.3390/v13030416
Abstract: Glycan–protein interactions are highly specific yet transient, rendering glycans ideal recognition signals in a variety of biological processes. In human norovirus (HuNoV) infection, histo-blood group antigens (HBGAs) play an essential but poorly understood role. For… read more here.
Keywords: capsid proteins; nmr experiments; recognition; glycan recognition ... See more keywords
Dengue and Zika Virus Capsid Proteins Contain a Common PEX19-Binding Motif
Sign Up to like & getrecommendations! Published in 2022 at "Viruses"
DOI: 10.3390/v14020253
Abstract: Flaviviruses such as dengue virus (DENV) and Zika virus (ZIKV) have evolved sophisticated mechanisms to suppress the host immune system. For instance, flavivirus infections were found to sabotage peroxisomes, organelles with an important role in… read more here.
Keywords: binding motif; pex19; capsid proteins; virus ... See more keywords
Flavivirus Capsid Proteins Inhibit the Interferon Response
Sign Up to like & getrecommendations! Published in 2022 at "Viruses"
DOI: 10.3390/v14050968
Abstract: Zika virus (ZIKV) establishes persistent infections in multiple human tissues, a phenomenon that likely plays a role in its ability to cause congenital birth defects and neurological disease. Multiple nonstructural proteins encoded by ZIKV, in… read more here.
Keywords: flavivirus capsid; response flavivirus; capsid proteins; ifn ... See more keywords