QM/MM Simulations Reveal the Determinants of Carbapenemase Activity in Class A β-Lactamases
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DOI: 10.1021/acsinfecdis.2c00152
Abstract: β-lactam antibiotic resistance in Gram-negative bacteria, primarily caused by β-lactamase enzymes that hydrolyze the β-lactam ring, has become a serious clinical problem. Carbapenems were formerly considered “last resort” antibiotics because they escaped breakdown by most… read more here.
Keywords: carbapenemase activity; class; enzyme; mechanics ... See more keywords
Structural basis for substrate specificity and carbapenemase activity of OXA-48 Class D β-lactamase.
Sign Up to like & getrecommendations! Published in 2019 at "ACS infectious diseases"
DOI: 10.1021/acsinfecdis.9b00304
Abstract: Carbapenem-hydrolyzing class D β-lactamases (CHDLs) are a diverse family of enzymes that are rapidly becoming the predominant cause of bacterial resistance against β-lactam antibiotics in many regions of the world. OXA-48, an atypical member of… read more here.
Keywords: carbapenemase activity; class; acyl enzyme; product ... See more keywords
Structural Insights into the Mechanism of Carbapenemase Activity of the OXA-48 β-Lactamase
Sign Up to like & getrecommendations! Published in 2019 at "Antimicrobial Agents and Chemotherapy"
DOI: 10.1128/aac.01202-19
Abstract: Carbapenem-hydrolyzing class D carbapenemases (CHDLs) are enzymes that produce resistance to the last-resort carbapenem antibiotics, severely compromising the available therapeutic options for the treatment of life-threatening infections. A broad variety of CHDLs, including OXA-23, OXA-24/40,… read more here.
Keywords: baumannii; mechanism carbapenemase; oxa; carbapenemase activity ... See more keywords
Role of the Hydrophobic Bridge in the Carbapenemase Activity of Class D β-Lactamases
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DOI: 10.1128/aac.02191-18
Abstract: Class D carbapenemases are enzymes of the utmost clinical importance due to their ability to confer resistance to the last-resort carbapenem antibiotics. We investigated the role of the conserved hydrophobic bridge in the carbapenemase activity… read more here.
Keywords: carbapenemase activity; class; carbapenemase; bridge ... See more keywords
False-Positive Carbapenem-Hydrolyzing Confirmatory Tests Due to ACT-28, a Chromosomally Encoded AmpC with Weak Carbapenemase Activity from Enterobacter kobei
Sign Up to like & getrecommendations! Published in 2019 at "Antimicrobial Agents and Chemotherapy"
DOI: 10.1128/aac.02388-18
Abstract: In Enterobacter cloacae complex (ECC), the overproduction of the chromosome-encoded cephalosporinase (cAmpC) associated with decreased outer membrane permeability may result in carbapenem resistance. In this study, we have characterized ACT-28, a cAmpC with weak carbapenemase… read more here.
Keywords: enterobacter; carbapenemase activity; act; carbapenemase ... See more keywords