Assigning Function to Phylogeny: FISH-nanoSIMS.
Sign Up to like & getrecommendations! Published in 2021 at "Methods in molecular biology"
DOI: 10.1007/978-1-0716-1115-9_13
Abstract: Catalyzed reporter deposition fluorescence in situ hybridization (CARD-FISH) is an imaging method used to identify microorganisms in environmental samples based on their phylogeny. CARD-FISH can be combined with nano-scale secondary ion mass spectrometry (nanoSIMS) to… read more here.
Keywords: assigning function; function phylogeny; fish nanosims; card fish ... See more keywords
Distinct relationships between fluorescence in situ hybridization and 16S rRNA gene- and amplicon-based sequencing data of bacterioplankton lineages.
Sign Up to like & getrecommendations! Published in 2019 at "Systematic and applied microbiology"
DOI: 10.1016/j.syapm.2019.06.005
Abstract: Catalyzed reporter deposition fluorescence in situ hybridization (CARD-FISH) and amplicon sequencing of the total (16S rRNA gene) and potentially active (16S rRNA transcripts), community are the major state of the art approaches for assessing the… read more here.
Keywords: sequencing data; bacterioplankton; amplicon; amplicon sequencing ... See more keywords
Rapid and Sensitive Quantification of Anammox Bacteria by Flow Cytometric Analysis Based on Catalyzed Reporter Deposition Fluorescence In Situ Hybridization.
Sign Up to like & getrecommendations! Published in 2019 at "Environmental science & technology"
DOI: 10.1021/acs.est.9b01017
Abstract: The quantification of anammox bacteria is crucial to manipulation and management of anammox biosystems. In this study, we proposed a protocol specifically optimized for quantification of anammox bacteria abundance in anammox sludge samples using catalyzed… read more here.
Keywords: anammox; fluorescence; quantification anammox; anammox bacteria ... See more keywords