SAXS reveals highly flexible interdomain linkers of tandem acyl carrier protein–thioesterase domains from a fungal nonreducing polyketide synthase
Sign Up to like & getrecommendations! Published in 2020 at "FEBS Letters"
DOI: 10.1002/1873-3468.13954
Abstract: Menisporopsin A is a fungal bioactive macrocyclic polylactone, the biosynthesis of which requires only reducing (R) and nonreducing (NR) polyketide synthases (PKSs) to guide a series of esterification and cyclolactonization reactions. There is no structural… read more here.
Keywords: nonreducing polyketide; carrier protein; acyl carrier; highly flexible ... See more keywords
The α/β Hydrolase AzpM Catalyzes Dipeptide Synthesis in Alazopeptin Biosynthesis Using Two Molecules of Carrier Protein‐Tethered Amino Acid
Sign Up to like & getrecommendations! Published in 2022 at "ChemBioChem"
DOI: 10.1002/cbic.202100700
Abstract: During the biosynthesis of alazopeptin, a tripeptide composed of two molecules of 6‐diazo‐5‐oxo‐L‐norleucine (DON) and one of alanine, the α/β hydrolase AzpM synthesizes the DON‐DON dipeptide using DON tethered to the carrier protein AzpF (DON‐AzpF).… read more here.
Keywords: azpf; two molecules; carrier protein; azpm ... See more keywords
Site‐Specific Multi‐Functionalization of the Carrier Protein CRM197 by Disulfide Rebridging for Conjugate Vaccine Development
Sign Up to like & getrecommendations! Published in 2022 at "Chembiochem"
DOI: 10.1002/cbic.202200408
Abstract: Conjugation of an antigen to a carrier protein is widely used for vaccine development. To develop the next generation of conjugate vaccines, we describe here a method for the controlled multi‐functionalization of the widely employed… read more here.
Keywords: carrier; functionalization; vaccine development; carrier protein ... See more keywords
Atomistic Insight on Structure and Dynamics of Spinach Acyl Carrier Protein with Substrate Length.
Sign Up to like & getrecommendations! Published in 2021 at "Biophysical journal"
DOI: 10.1016/j.bpj.2020.12.036
Abstract: The plant acyl-acyl carrier protein (ACP) desaturases are a family of soluble enzymes that convert saturated fatty-acyl ACPs into their cis-monounsaturated equivalents in an oxygen-dependent reaction. These enzymes play a key role in biosynthesis of… read more here.
Keywords: structure dynamics; carrier protein; acyl carrier; acyl ... See more keywords
Design and synthesis of thiourea-based derivatives as Mycobacterium tuberculosis growth and enoyl acyl carrier protein reductase (InhA) inhibitors.
Sign Up to like & getrecommendations! Published in 2020 at "European journal of medicinal chemistry"
DOI: 10.1016/j.ejmech.2020.112402
Abstract: Tuberculosis remains the most deadly infectious disease worldwide due to the emergence of drug-resistant strains of Mycobacterium tuberculosis. Hence, there is a great need for more efficient treatment regimens. Herein, we carried out rational molecular… read more here.
Keywords: carrier protein; protein reductase; acyl carrier; tuberculosis ... See more keywords
L. major apo-acyl carrier protein forms ordered aggregates due to an exposed phenylalanine, while phosphopantetheine inhibits aggregation in the holo-form.
Sign Up to like & getrecommendations! Published in 2021 at "International journal of biological macromolecules"
DOI: 10.1016/j.ijbiomac.2021.02.215
Abstract: L. major acyl carrier protein (ACP) is a mitochondrial protein, involved in fatty acid biosynthesis. The protein is expressed as an apo-protein, and post-translationally modified at Ser 37 by a 4'-Phosphopantetheinyl transferase. Crystal structure of… read more here.
Keywords: protein; carrier protein; protein forms; acyl carrier ... See more keywords
Development of meningococcal polysaccharide conjugate vaccine that can elicit long-lasting and strong cellular immune response with hepatitis B core antigen virus-like particles as a novel carrier protein.
Sign Up to like & getrecommendations! Published in 2019 at "Vaccine"
DOI: 10.1016/j.vaccine.2018.12.073
Abstract: Neisseria meningitidis caused meningitis is life-threatening acute infection with high fatality and high frequency of severe sequelae. Meningococcal capsular polysaccharides can be used to prevent meningococcal disease; while conjugating the polysaccharides to carrier protein was… read more here.
Keywords: carrier protein; conjugate; hbc; carrier ... See more keywords
Development of a bivalent conjugate vaccine candidate against rotaviral diarrhea and tuberculosis using polysaccharide from Mycobacterium tuberculosis conjugated to ΔVP8* protein from rotavirus.
Sign Up to like & getrecommendations! Published in 2021 at "Vaccine"
DOI: 10.1016/j.vaccine.2021.09.067
Abstract: Conjugation of carbohydrate antigens with a carrier protein is a clinically proven strategy to overcome the poor immunogenicity of bacterial polysaccharide. In addition to its primary role, which is to help generate a T cell-mediate… read more here.
Keywords: vaccine; carrier protein; tuberculosis; mycobacterium tuberculosis ... See more keywords
Solution Structure and Conformational Dynamics of a Doublet Acyl Carrier Protein from Prodigiosin Biosynthesis.
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DOI: 10.1021/acs.biochem.0c00830
Abstract: The acyl carrier protein (ACP) is an indispensable component of both fatty acid and polyketide synthases and is primarily responsible for delivering acyl intermediates to enzymatic partners. At present, increasing numbers of multidomain ACPs have… read more here.
Keywords: protein; carrier protein; acp; acyl carrier ... See more keywords
Tracking carrier protein motions with Raman spectroscopy
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DOI: 10.1038/s41467-019-10184-2
Abstract: Engineering microbial biosynthetic pathways represents a compelling route to gain access to expanded chemical diversity. Carrier proteins (CPs) play a central role in biosynthesis, but the fast motions of CPs make their conformational dynamics difficult… read more here.
Keywords: carrier protein; carrier; tracking carrier; protein motions ... See more keywords
Molecular basis for interactions between an acyl carrier protein and a ketosynthase
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DOI: 10.1038/s41589-019-0301-y
Abstract: Fatty acid synthases are dynamic ensembles of enzymes that can biosynthesize long hydrocarbon chains efficiently. Here we visualize the interaction between the Escherichia coli acyl carrier protein (AcpP) and β-ketoacyl-ACP-synthase I (FabB) using X-ray crystallography,… read more here.
Keywords: protein; carrier protein; interactions acyl; molecular basis ... See more keywords