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Published in 2020 at "Chemical communications"
DOI: 10.1039/d0cc01174f
Abstract: We describe a versatile CRISPR/Cas-based strategy to construct multi-enzyme complexes scaffolded on a DNA template in programmable patterns. Catalytically inactive dCas9 nuclease was used in combination with SpyCatcher-SpyTag chemistry to assemble enzymes in a highly…
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Keywords:
multi enzyme;
crispr cas;
enzyme complexes;
cas directed ... See more keywords