The anti-CRISPR protein AcrIE8.1 inhibits the type I-E CRISPR-Cas system by directly binding to the Cascade subunit Cas11.
Sign Up to like & getrecommendations! Published in 2025 at "FEBS letters"
DOI: 10.1002/1873-3468.70201
Abstract: CRISPR-Cas systems provide adaptive immunity to bacteria by recognizing and destroying foreign genetic elements. The type I-E CRISPR-Cas system utilizes a multi-subunit Cascade complex to detect target DNA and recruit the Cas3 nuclease for degradation.… read more here.
Keywords: crispr; cas system; subunit; crispr cas ... See more keywords
Genome Editing Using the Endogenous Type I-E CRISPR-Cas System in Lactobacillus paracasei ATCC334.
Sign Up to like & getrecommendations! Published in 2025 at "Biotechnology and applied biochemistry"
DOI: 10.1002/bab.70056
Abstract: Lactobacillus paracasei ATCC334 is a well-known beneficial strain that plays a crucial role in food industry and promotion of human health. However, despite its significance, our understanding of its gene functions remains limited due to… read more here.
Keywords: system; cas system; paracasei atcc334; crispr cas ... See more keywords
CRISPR-Cas system manipulating nanoparticles signal transduction for cancer diagnosis.
Sign Up to like & getrecommendations! Published in 2022 at "Wiley interdisciplinary reviews. Nanomedicine and nanobiotechnology"
DOI: 10.1002/wnan.1851
Abstract: Early diagnosis of cancer is important to improve the survival rate and relieve patient pain. Sensitive detection of cancer related biomarkers in body fluids is a critical approach for the early diagnosis of cancer. The… read more here.
Keywords: diagnosis; crispr cas; cas system; cancer ... See more keywords
Correlation between type IIIA CRISPR-Cas system and SCCmec in Staphylococcus epidermidis.
Sign Up to like & getrecommendations! Published in 2021 at "Archives of microbiology"
DOI: 10.1007/s00203-021-02595-x
Abstract: A subculture of S.epidermidis strain ATCC35984 that is amenable to genetically manipulate was occasionally found in our laboratory. This mutant exhibited susceptibility to methicillin in contrast to its parent strain. To unveil the underlying mechanism,… read more here.
Keywords: crispr cas; cas; system sccmec; cas system ... See more keywords
Application of the CRISPR/Cas system for genome editing in microalgae
Sign Up to like & getrecommendations! Published in 2019 at "Applied Microbiology and Biotechnology"
DOI: 10.1007/s00253-019-09726-x
Abstract: Microalgae are arguably the most abundant single-celled eukaryotes and are widely distributed in oceans and freshwater lakes. Moreover, microalgae are widely used in biotechnology to produce bioenergy and high-value products such as polyunsaturated fatty acids… read more here.
Keywords: genome editing; crispr cas; cas system;
Genomic analysis of multi-drug resistant coagulase-negative staphylococci from healthy humans and animals revealed unusual mechanisms of resistance and CRISPR-Cas system
Sign Up to like & getrecommendations! Published in 2024 at "International Microbiology"
DOI: 10.1007/s10123-024-00577-9
Abstract: Coagulase-negative staphylococci (CoNS) are evolving as major reservoirs and vectors of unusual and critical antimicrobial resistance (AMR) mechanisms. In this study, the genomic characterization of 26 multidrug-resistant (MDR)-CoNS (S. borealis, S. saprophyticus, S. sciuri, S.… read more here.
Keywords: resistance; negative staphylococci; crispr cas; coagulase negative ... See more keywords
Recent advances in CRISPR technologies for genome editing.
Sign Up to like & getrecommendations! Published in 2021 at "Archives of pharmacal research"
DOI: 10.1007/s12272-021-01336-4
Abstract: The discovery of clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein (Cas) system, and its development into a set of powerful tools for manipulating the genome, has revolutionized genome editing. Precise, targeted CRISPR/Cas-based genome editing… read more here.
Keywords: genome editing; crispr cas; cas; cas system ... See more keywords
CRISPR-Cas system, antibiotic resistance and virulence in bacteria: Through a common lens.
Sign Up to like & getrecommendations! Published in 2021 at "Progress in molecular biology and translational science"
DOI: 10.1016/bs.pmbts.2020.12.005
Abstract: CRISPR-Cas system, antibiotic resistance and virulence are different modes of survival for the bacteria. CRISPR-Cas is an adaptive immune system that can degrade foreign DNA, antibiotic resistance helps bacteria to evade drugs that can threaten… read more here.
Keywords: system; virulence; crispr cas; resistance ... See more keywords
Advances in amplification-free detection of nucleic acid: CRISPR/Cas system as a powerful tool.
Sign Up to like & getrecommendations! Published in 2022 at "Analytical biochemistry"
DOI: 10.1016/j.ab.2022.114593
Abstract: Amplification technologies such as polymerase chain reaction (PCR) play an important role in nucleic acid detection. However, they require bulky and sophisticated thermal cycling instrument, as well as are prone to get false-positive results due… read more here.
Keywords: crispr cas; amplification; detection; cas system ... See more keywords
Insights to improve the plant nutrient transport by CRISPR/Cas system.
Sign Up to like & getrecommendations! Published in 2022 at "Biotechnology advances"
DOI: 10.1016/j.biotechadv.2022.107963
Abstract: We need to improve food production to feed the ever growing world population especially in a changing climate. Nutrient deficiency in soils is one of the primary bottlenecks affecting the crop production both in developed… read more here.
Keywords: cas system; plant nutrient; nutrient transport; transport ... See more keywords
Targeted mutagenesis of Mycoplasma gallisepticum using its endogenous CRISPR/Cas system.
Sign Up to like & getrecommendations! Published in 2020 at "Veterinary microbiology"
DOI: 10.1016/j.vetmic.2020.108868
Abstract: New, more efficient methods are needed to facilitate studies of gene function in the mycoplasmas. CRISPR/Cas systems, which provide bacteria with acquired immunity against invading nucleic acids, have been developed as tools for genomic editing… read more here.
Keywords: crispr cas; gallisepticum; crispr; cas system ... See more keywords