CRISPR/Cas Systems in Genome Editing: Methodologies and Tools for sgRNA Design, Off‐Target Evaluation, and Strategies to Mitigate Off‐Target Effects
Sign Up to like & getrecommendations! Published in 2020 at "Advanced Science"
DOI: 10.1002/advs.201902312
Abstract: Abstract Life sciences have been revolutionized by genome editing (GE) tools, including zinc finger nucleases, transcription activator‐Like effector nucleases, and CRISPR (clustered regulatory interspaced short palindromic repeats)/Cas (CRISPR‐associated) systems, which make the targeted modification of… read more here.
Keywords: genome editing; target effects; crispr cas; target ... See more keywords
Gold Nanomaterials-Implemented CRISPR-Cas Systems for Biosensing.
Sign Up to like & getrecommendations! Published in 2023 at "Small"
DOI: 10.1002/smll.202300057
Abstract: Due to their superiority in the simple design and precise targeting, clustered regularly interspaced short palindromic repeats (CRISPR)-Cas systems have attracted significant interest for biosensing. On the one hand, CRISPR-Cas systems have the capacity to… read more here.
Keywords: gold nanomaterials; crispr cas; cas systems;
CRISPR/Cas Systems‐Inspired Nano/Biosensors for Detecting Infectious Viruses and Pathogenic Bacteria
Sign Up to like & getrecommendations! Published in 2022 at "Small Methods"
DOI: 10.1002/smtd.202200794
Abstract: Infectious pathogens cause severe human illnesses and great deaths per year worldwide. Rapid, sensitive, and accurate detection of pathogens is of great importance for preventing infectious diseases caused by pathogens and optimizing medical healthcare systems.… read more here.
Keywords: nano biosensors; infectious viruses; pathogenic bacteria; viruses pathogenic ... See more keywords
Methods for the Analysis and Characterization of Defense Mechanisms Against Horizontal Gene Transfer: CRISPR Systems.
Sign Up to like & getrecommendations! Published in 2020 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-9877-7_17
Abstract: CRISPR-Cas systems provide RNA-guided adaptive immunity to the majority of archaea and many bacteria. They are able to capture pieces of invading genetic elements in the form of novel spacers in an array of repeats.… read more here.
Keywords: gene transfer; crispr cas; cas systems; horizontal gene ... See more keywords
Insights of CRISPR-Cas systems in stem cells: progress in regenerative medicine.
Sign Up to like & getrecommendations! Published in 2021 at "Molecular biology reports"
DOI: 10.1007/s11033-021-06832-w
Abstract: Regenerative medicine, a therapeutic approach using stem cells, aims to rejuvenate and restore the normalized function of the cells, tissues, and organs that are injured, malfunctioning, and afflicted. This influential technology reaches its zenith when… read more here.
Keywords: medicine; crispr cas; cas systems; stem cells ... See more keywords
An introduction to CRISPR-Cas systems for reprogramming the genome of mammalian cells.
Sign Up to like & getrecommendations! Published in 2021 at "Progress in molecular biology and translational science"
DOI: 10.1016/bs.pmbts.2021.01.010
Abstract: In the past few decades, it has been possible to introduce unprecedented mutations in genes of the mammalian cells owing to the development of advanced technologies/methods/assays. Sometimes, these mutations occurring at the cellular level may… read more here.
Keywords: cas systems; introduction crispr; mammalian cells; crispr cas ... See more keywords
Harnessing CRISPR/Cas systems for programmable transcriptional and post-transcriptional regulation.
Sign Up to like & getrecommendations! Published in 2018 at "Biotechnology advances"
DOI: 10.1016/j.biotechadv.2017.11.008
Abstract: Genome editing has enabled broad advances and novel approaches in studies of gene function and structure; now, emerging methods aim to precisely engineer post-transcriptional processes. Developing precise, efficient molecular tools to alter the transcriptome holds… read more here.
Keywords: systems programmable; crispr cas; post transcriptional; cas systems ... See more keywords
SnapShot: Class 2 CRISPR-Cas Systems
Sign Up to like & getrecommendations! Published in 2017 at "Cell"
DOI: 10.1016/j.cell.2016.12.038
Abstract: Class 2 CRISPR-Cas systems are characterized by effector modules consisting of single, large, multidomain proteins that appear to have been derived from mobile genetic elements. Some Class 2 effector proteins, such as Cas9 and Cas12a… read more here.
Keywords: class; crispr cas; cas systems; class crispr ... See more keywords
Harnessing CRISPR-Cas systems for precision engineering of designer probiotic lactobacilli.
Sign Up to like & getrecommendations! Published in 2019 at "Current opinion in biotechnology"
DOI: 10.1016/j.copbio.2018.11.009
Abstract: Our evolving understanding on the mechanisms underlying the health-promoting attributes of probiotic lactobacilli, together with an expanding genome editing toolbox have made this genus an ideal chassis for the development of living therapeutics. The rising… read more here.
Keywords: designer; engineering; crispr cas; lactobacilli ... See more keywords
Engineering virus resistance via CRISPR-Cas systems.
Sign Up to like & getrecommendations! Published in 2018 at "Current opinion in virology"
DOI: 10.1016/j.coviro.2018.06.002
Abstract: In prokaryotes, CRISPR/Cas adaptive immunity systems target and destroy nucleic acids derived from invading bacteriophages and other foreign genetic elements. In eukaryotes, the native function of these systems has been exploited to combat viruses in… read more here.
Keywords: resistance; crispr cas; cas systems; virus ... See more keywords
Drug Inducible CRISPR/Cas Systems
Sign Up to like & getrecommendations! Published in 2019 at "Computational and Structural Biotechnology Journal"
DOI: 10.1016/j.csbj.2019.07.015
Abstract: Clustered, regularly interspaced, short palindromic repeats (CRISPR)/CRISPR-associated protein (Cas) systems have been employed as a powerful versatile technology for programmable gene editing, transcriptional modulation, epigenetic modulation, and genome labeling, etc. Yet better control of their… read more here.
Keywords: inducible crispr; crispr cas; drug inducible; cas ... See more keywords