Selective loading and processing of prespacers for precise CRISPR adaptation
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DOI: 10.1038/s41586-020-2018-1
Abstract: CRISPR–Cas immunity protects prokaryotes against invading genetic elements 1 . It uses the highly conserved Cas1–Cas2 complex to establish inheritable memory (spacers) 2 – 5 . How Cas1–Cas2 acquires spacers from foreign DNA fragments (prespacers)… read more here.
Keywords: pam sequence; correct orientation; integration; cas1 cas2 ... See more keywords
Global phylogenomic novelty of the Cas1 gene from hot spring microbial communities
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DOI: 10.3389/fmicb.2022.1069452
Abstract: The Cas1 protein is essential for the functioning of CRISPR-Cas adaptive systems. However, despite the high prevalence of CRISPR-Cas systems in thermophilic microorganisms, few studies have investigated the occurrence and diversity of Cas1 across hot… read more here.
Keywords: spring microbial; cas1; hot spring; microbial communities ... See more keywords
DNA Motifs and an Accessory CRISPR Factor Determine Cas1 Binding and Integration Activity in Sulfolobus islandicus
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DOI: 10.3390/ijms231710178
Abstract: CRISPR-Cas systems empower prokaryotes with adaptive immunity against invasive mobile genetic elements. At the first step of CRISPR immunity adaptation, short DNA fragments from the invaders are integrated into CRISPR arrays at the leader-proximal end.… read more here.
Keywords: crispr; cas1; leader; leader proximal ... See more keywords