Target recognition triggered CRISPR-Cas12a assisted allosteric scaffold for sensitively analyzing bacterial infection after dental implantation.
Sign Up to like & getrecommendations! Published in 2022 at "Analytical biochemistry"
DOI: 10.1016/j.ab.2022.114666
Abstract: Accurate identification and sensitive quantification of infected bacteria after dental implant both play crucial roles in early-diagnosis of bacterial infection and guiding medicine applications. Herein, we propose a sensitive and accurate bacteria detection method based… read more here.
Keywords: bacterial infection; assisted allosteric; crispr cas12a; allosteric scaffold ... See more keywords
CRISPR-Cas12a-based efficient electrochemiluminescence biosensor for ATP detection.
Sign Up to like & getrecommendations! Published in 2021 at "Analytica chimica acta"
DOI: 10.1016/j.aca.2021.339180
Abstract: CRISPR-Cas12a system exhibits tremendous potential in accurate recognition and quantitation of nucleic acids and non-nucleic-acid targets thanks to the discovery of its cleavage capability toward single-stranded DNA (ssDNA). In this study, we developed an efficient… read more here.
Keywords: crispr cas12a; detection; crispr; cas12a ... See more keywords
Potential application of CRISPR-Cas12a fluorescence assay coupled with rapid nucleic acid amplification for detection of white spot syndrome virus in shrimp
Sign Up to like & getrecommendations! Published in 2019 at "Aquaculture"
DOI: 10.1016/j.aquaculture.2019.734340
Abstract: Abstract White spot disease (WSD), caused by white spot syndrome virus (WSSV), is among the most severe diseases of cultivated shrimp. Here, the CRISPR-Cas12a system coupled with nucleic acid amplification was optimized for the detection… read more here.
Keywords: amplification; cas12a; crispr cas12a; white spot ... See more keywords
Enzymatic Recombinase Amplification Coupled with CRISPR-Cas12a for Ultrasensitive, Rapid, and Specific Porcine circovirus 3 Detection.
Sign Up to like & getrecommendations! Published in 2021 at "Molecular and cellular probes"
DOI: 10.1016/j.mcp.2021.101763
Abstract: Porcine circovirus type 3 (PCV3) is a disease associated with porcine dermatitis and nephrotic syndrome (PDNS) that has caused significant economic losses to swine herds since its discovery in 2016. To develop a simple, on-site,… read more here.
Keywords: crispr cas12a; cas12a; porcine circovirus; enzymatic recombinase ... See more keywords
Accurate and sensitive analysis of Staphylococcus aureus through CRISPR-Cas12a based recycling signal amplification cascades for early diagnosis of skin and soft tissue infections.
Sign Up to like & getrecommendations! Published in 2021 at "Journal of microbiological methods"
DOI: 10.1016/j.mimet.2021.106167
Abstract: Early-diagnosis and treatments of skin and soft tissue infections remain a huge challenge due to the difficulties in the detection of trace amounts of bacteria. We develop here a novel method through CRISPR-Cas12a based recycling… read more here.
Keywords: crispr cas12a; skin soft; soft tissue; cas12a ... See more keywords
A new CRISPR-derived microRNA sensing mechanism based on Cas12a self-powered and rolling circle transcription (RCT)-unleashed real-time crRNA recruiting.
Sign Up to like & getrecommendations! Published in 2020 at "Analytical chemistry"
DOI: 10.1021/acs.analchem.0c00680
Abstract: Current CRISPR-Cas-based nucleic acid sensing methods relying on the pre-assembled Cas-crRNA complexes are gen-erally limited to the detection of protospacer-adjacent motif (PAM)-containing sequences, and nonspecific back-grounds are inevitable. Herein, we propose a new CRISPR-derived microRNA… read more here.
Keywords: crispr; rct; cas12a; crispr derived ... See more keywords
Reverse Transcription Recombinase Polymerase Amplification Coupled with CRISPR-Cas12a for Facile and Highly Sensitive Colorimetric SARS-CoV-2 Detection
Sign Up to like & getrecommendations! Published in 2021 at "Analytical Chemistry"
DOI: 10.1021/acs.analchem.1c00013
Abstract: The outbreak of the pandemic caused by the severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) calls for an urgent unmet need for developing a facial and cost-effective detection method. The requirement of well-trained personnel and sophisticated… read more here.
Keywords: detection; cas12a; reverse transcription; sars cov ... See more keywords
Integration of the Ligase Chain Reaction with the CRISPR-Cas12a System for Homogeneous, Ultrasensitive, and Visual Detection of microRNA.
Sign Up to like & getrecommendations! Published in 2022 at "Analytical chemistry"
DOI: 10.1021/acs.analchem.2c00294
Abstract: The ligase chain reaction (LCR), as a classic nucleic acid amplification technique, is popular in the detection of DNA and RNA due to its simplicity, powerfulness, and high specificity. However, homogeneous and ultrasensitive LCR detection… read more here.
Keywords: detection; cas12a; crispr cas12a; cas12a system ... See more keywords
CRISPR-Cas12a-Derived Photoelectrochemical Biosensor for Point-Of-Care Diagnosis of Nucleic Acid.
Sign Up to like & getrecommendations! Published in 2022 at "Analytical chemistry"
DOI: 10.1021/acs.analchem.2c01373
Abstract: This work presented a point-of-care (POC) photoelectrochemical (PEC) biosensing for the detection of human papillomavirus-16 (HPV-16) on a portable electrochemical detection system by using CRISPR-Cas12a trans-cleaving the G-quadruplex for the biorecognition/amplification and a hollow In2O3-In2S3-modified… read more here.
Keywords: point care; crispr cas12a; cas12a; photocurrent ... See more keywords
"RESET" Effect: Random Extending Sequences Enhance the Trans-Cleavage Activity of CRISPR/Cas12a.
Sign Up to like & getrecommendations! Published in 2022 at "Analytical chemistry"
DOI: 10.1021/acs.analchem.2c01401
Abstract: The trans-cleavage activity of CRISPR/Cas12a has been widely used in biosensing applications. However, the lack of exploration on the fundamental properties of CRISPR/Cas12a not only discourages further in-depth studies of the CRISPR/Cas12a system but also… read more here.
Keywords: crispr cas12a; trans cleavage; cas12a; cleavage activity ... See more keywords
Kinetics Accelerated CRISPR-Cas12a Enabling Live-Cell Monitoring of Mn2+ Homeostasis.
Sign Up to like & getrecommendations! Published in 2022 at "Analytical chemistry"
DOI: 10.1021/acs.analchem.2c01461
Abstract: The CRISPR/Cas12a system has been repurposed as a versatile nuclei acid bio-imaging tool, but its utility in sensing non-nucleic acid analytes in living cells has been less exploited. Herein, we demonstrated the ability of Mn2+… read more here.
Keywords: live cell; cas12a; mn2; crispr cas12a ... See more keywords