CRISPR-Cas12a-Based Diagnostics of Wheat Fungal Diseases.
Sign Up to like & getrecommendations! Published in 2022 at "Journal of agricultural and food chemistry"
DOI: 10.1021/acs.jafc.1c08391
Abstract: Fusarium head blight (FHB) of wheat, mainly caused by Fusarium graminearum (F. graminearum) infection, reduces crop yield and contaminates grain with mycotoxins. We report a clustered regularly interspaced short palindromic repeats (CRISPR)-Cas12a-based nucleic acid assay… read more here.
Keywords: cas12a based; based diagnostics; diagnostics wheat; wheat fungal ... See more keywords
CRISPR-Cas12a based internal negative control for nonspecific products of exponential rolling circle amplification
Sign Up to like & getrecommendations! Published in 2020 at "Nucleic Acids Research"
DOI: 10.1093/nar/gkaa017
Abstract: Abstract False-positive results cause a major problem in nucleic acid amplification, and require external blank/negative controls for every test. However, external controls usually have a simpler and lower background compared to the test sample, resulting… read more here.
Keywords: cas12a based; based internal; rolling circle; exponential rolling ... See more keywords
Development and clinical application of a novel CRISPR-Cas12a based assay for the detection of African swine fever virus
Sign Up to like & getrecommendations! Published in 2020 at "BMC Microbiology"
DOI: 10.1186/s12866-020-01966-6
Abstract: Background As no treatment or effective vaccine for African swine fever virus (ASFV) is currently available, a rapid, highly sensitive diagnostic is urgently needed to curb the spread of ASFV. Results Here we designed a… read more here.
Keywords: based assay; crispr cas12a; virus; cas12a based ... See more keywords
A CRISPR/Cas12a-based portable platform for rapid detection of Leptosphaeria maculans in Brassica crops
Sign Up to like & getrecommendations! Published in 2022 at "Frontiers in Plant Science"
DOI: 10.3389/fpls.2022.976510
Abstract: Establishing a portable diagnostic method for identifying plant pathogens is essential to prevent the spread of plant disease, especially in field and customs inspections. Leptosphaeria maculans (L. maculans) is an aggressive fungus, which causes severe… read more here.
Keywords: based portable; cas12a based; detection; leptosphaeria maculans ... See more keywords