Regulating cleavage activity and enabling microRNA detection with split sgRNA in Cas12b
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DOI: 10.1038/s41467-025-61748-4
Abstract: The CRISPR-Cas12b system has revolutionized molecular diagnostics, yet its reliance on single guide RNAs (sgRNAs) exceeding 100 nt limits precise regulation and applications. We present a split sgRNA strategy for Cas12b, utilizing universal components with… read more here.
Keywords: split; split sgrna; microrna detection; cas12b ... See more keywords