Therapeutic In Vivo Gene Editing Achieved by a Hypercompact CRISPR‐Cas12f1 System Delivered with All‐in‐One Adeno‐Associated Virus
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DOI: 10.1002/advs.202308095
Abstract: CRISPR‐based gene therapies are making remarkable strides toward the clinic. But the large size of most widely used Cas endonucleases including Cas9 and Cas12a restricts their efficient delivery by the adeno‐associated virus (AAV) for in… read more here.
Keywords: system; gene; vivo gene; cas12f1 ... See more keywords
Comparison of CRISPR-Cas9, CRISPR-Cas12f1, and CRISPR-Cas3 in eradicating resistance genes KPC-2 and IMP-4
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DOI: 10.1128/spectrum.02572-24
Abstract: ABSTRACT Bacterial plasmid encoding antibiotic resistance could be eradicated by various CRISPR systems, such as CRISPR-Cas9, Cas12f1, and Cas3. However, the efficacy of these gene editing tools against bacterial resistance has not been systematically assessed… read more here.
Keywords: cas3; kpc imp; resistance; cas12f1 ... See more keywords
Single nucleotide genome recognition and selective bacterial lysis using synthetic phages loaded with CRISPR-Cas12f1-truncated sgRNA.
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DOI: 10.71150/jm.2501012
Abstract: Phage specificity primarily relies on host cell-surface receptors. However, integrating cas genes and guide RNAs into phage genomes could enhance their target specificity and regulatory effects. In this study, we developed a CRISPR-Cas12f1 system-equipped bacteriophage… read more here.
Keywords: cas12f1 truncated; crispr cas12f1; single nucleotide; synthetic phages ... See more keywords